α-Amylase Activity Assay Kit
Référence E-BC-K007-M-96T
Conditionnement : 96T
Marque : Elabscience
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
For research use only.
| Detection Principle | The reducing sugar reacts with 3,5-dinitrosalicylic acid under heating conditions to produce a brown-red substance, which is inactivated by the thermolabile nature of β-amylase, and then the enzyme activity of α-amylase is determined. |
| Sample Type | Animal tissue,Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (535-540 nm) |
| Research Area | Plant Physiology Series |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.0097 U/mL |
| Detection Range | 0.0097-0.3474 U/mL |
| Precision | inter-assay CV: 3.9% | intra-assay CV: 3.0% |
| Sample Volume | 150 μL |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 1% Epipremnum aureum tissue homogenate | 1 |
| 1% Green pepper tissue homogenate | 1 |
| 1% Corn grain tissue homogenate | 1 |
| 1% Daucus carota tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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