0.25% Trypsin-EDTA, phenol red (1×)

Biorbyt’s 0.25% Trypsin-EDTA, Phenol Red (1×) is a ready-to-use cell dissociation solution specifically designed for routine passaging, harvesting, and single-cell preparation of adherent cells. The formulation contains trypsin and EDTA, which effectively disrupt cell–substrate and cell–cell interactions, enabling rapid and efficient cell detachment. The inclusion of phenol red serves as a pH indicator, allowing real-time monitoring of the dissociation process.This optimized formulation ensures efficient cell detachment while maximally preserving cell membrane integrity and biological activity, making it suitable for a wide range of mammalian adherent cell types. Supplied as a 1× working solution, the product can be used directly without dilution. It is ideal for routine cell culture applications, cell passaging, and pre-freezing preparation, and is an essential, commonly used reagent in cell culture laboratories.