1,2-Dioleoyl-3-trimethylammonium-propane, Chloride (DOTAP) [132172-61-3]

Cationic liposome-forming compound for transfection of DNA, RNA and other negatively charged molecules into eukaryotic cells. Mixing DOTAP with DNA results in spontaneously formed stable complexes that can be directly added to cell culture medium. These complexes fuse with the cell membrane and release DNA into the cytoplasm. The cells are transfected efficiently without cytotoxic effects. Use approximately 5-10ug DOTAP per ug DNA and approximately 10-20ug DOTAP per ml cell culture medium. The optimal working concentration and transfection time depends in part on the cell line used, and the type of material (RNA, DNA, protein) loaded.

Synonyms:
DOTAP chloride

CAS No:
132172-61-3

Molecular Formula:
C42H80ClNO4

Molecular Weight:
698.55

Appearance:
Yellow to pale beige sticky solid.

Purity:
≥96%

Solubility:
Chloroform (Slightly), Methanol (Sparingly)

Method for Determining Identity:
Proton (CDCl3) NMR and Mass Spectroscopic Analysis

TLC Conditions:
SiO2; Dichloromethane: Methanol=8:2; Visualized with UV and KMnO4; Single spot; Rf=0.70

Chloride:
As reported

Atmosphere:
Inert gas

Storage and Stability:
May be stored at RT for short-term only. Long-term storage is recommended at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Hygroscopic. Store under inert atmosphere.