10x ELISA WASH BUFFER
Référence MBS238278-500mL
Conditionnement : 500mL
Marque : MyBiosource
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10x ELISA WASH BUFFER Accessory Reagent – 10x concentrate - liquid
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS238278MBS238278 is an accessory reagent targeting 10x ELISA WASH BUFFER. More details
Product Overview
Online Datasheet in PDFProduct Name
10x ELISA WASH BUFFER, Accessory Reagent
Full Product Name
10x ELISA WASH BUFFER
Form/Format
10x concentrate - liquid
Preparation and Storage
Store at 4°C.
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
Perservative Stabilizers
1.0% 2-chloroacetamide
Intended Use
ELISA wash buffer was developed as a high performance washing solution for use in all ELISA formats. The optimised formulation of pH stabilizers, salts and detergents increases the efficiency of washing stages during ELISA, leading to a reduction in background noise and an increased specific signal.
MBS238278 is compatible with all routinely used conjugate components such as HRP, Avidin and Alkaline Phosphatase.
MBS238278 is compatible with all routinely used conjugate components such as HRP, Avidin and Alkaline Phosphatase.
Instructions For Use
1. Dilute the required volume of this item 1:10 with deionised water (1 part This item to 9 parts water) and mix.
2. Use at each ELISA wash step for a total of 2-4 washes. Fill each well with the diluted wash buffer (approximately 400ul), taking care not to submerge the plate as this can cross-contaminate the wells. The buffer may be dispensed manually or by an automated system.
3. Between washes, aspirate or dump the buffer. After the final wash blot the plate on paper towels to remove any excess liquid.
2. Use at each ELISA wash step for a total of 2-4 washes. Fill each well with the diluted wash buffer (approximately 400ul), taking care not to submerge the plate as this can cross-contaminate the wells. The buffer may be dispensed manually or by an automated system.
3. Between washes, aspirate or dump the buffer. After the final wash blot the plate on paper towels to remove any excess liquid.
Buffer Solution
Phosphate buffered saline
Hazard
Harmful Substance
Applications Tested/Suitable for 10x ELISA WASH BUFFER accessory reagent
ELISA (EIA)
Application Notes for 10x ELISA WASH BUFFER accessory reagent
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Other Notes
Small volumes of 10x ELISA WASH BUFFER accessory reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.