Orb420112 | 10X RIPA Lysis Buffer Biovalley

10X RIPA Lysis Buffer

SKU: orb420112

Description

10X RIPA Lysis Buffer

Images & Validation

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Application Notes	Tested Applications: ChIP, IP, WB. This product is 10X concentrated stock solution. Dilute to 1X prior to use. 1X RIPA Lysis Buffer is intended for the extraction of cellular proteins for the efficient lysis of cells and solubilization of protein, while minimizing protein degradation and maintaining protein immunoreactivity and biological activity. We recommend using 1.0 ml of RIPA Lysis Buffer to lyse 0.5 to 5 x 10E7 adherent mammalian cells. This buffer contains ionic detergents and may not be suitable for kinase enzymes, if these enzymes are easily denatured. Do not add phosphatase inhibitors when preparing lysates for phosphatase assays. 1X RIPA lysis buffer consists of 50 mM Tris HCl, 150 mM NaCl, 1.0% (v/v) NP-40, 0.5% (w/v) Sodium Deoxycholate, 1.0 mM EDTA, 0.1% (w/v) SDS and 0.01% (w/v) sodium azide at a pH

Application Notes

Tested Applications: ChIP, IP, WB. This product is 10X concentrated stock solution. Dilute to 1X prior to use. 1X RIPA Lysis Buffer is intended for the extraction of cellular proteins for the efficient lysis of cells and solubilization of protein, while minimizing protein degradation and maintaining protein immunoreactivity and biological activity. We recommend using 1.0 ml of RIPA Lysis Buffer to lyse 0.5 to 5 x 10E7 adherent mammalian cells. This buffer contains ionic detergents and may not be suitable for kinase enzymes, if these enzymes are easily denatured. Do not add phosphatase inhibitors when preparing lysates for phosphatase assays. 1X RIPA lysis buffer consists of 50 mM Tris HCl, 150 mM NaCl, 1.0% (v/v) NP-40, 0.5% (w/v) Sodium Deoxycholate, 1.0 mM EDTA, 0.1% (w/v) SDS and 0.01% (w/v) sodium azide at a pH

Key Properties

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Purity	This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
Conjugation	Unconjugated

Storage & Handling

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Storage	Store container at room temperature (18° to 26° C) prior to opening. Protect from light (store in the dark).
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: See application note.
Concentration	10X
Expiration Date	12 months from date of receipt.
Hazard Information	Non-Toxic
Disclaimer	For research use only

Alternative Names

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10X RIPA Lysis Buffer, RIPA (Radio-Immunoprecipitation Assay) Lysis Buffer

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RadioImmunoPrecipitation Assay (RIPA) Buffer, 10X [orb2652890]
Applications:
IHC
Sizes Available: 500 ml
RadioImmunoPrecipitation Assay (RIPA) Buffer, 10X [orb2652891]
Applications:
IHC
Sizes Available: 100 ml
10X RIPA Lysis Buffer [orb420113]
Applications:
ChIP, IP, WB
Sizes Available: 50 ml

Protocol Information

Western Blot (IB, immunoblot)

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Immunoprecipitation

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ChIP

Chromatin Immunoprecipitation

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10X RIPA Lysis Buffer

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10X RIPA Lysis Buffer

Description

Images & Validation

Key Properties

Storage & Handling

Alternative Names

RadioImmunoPrecipitation Assay (RIPA) Buffer, 10X [orb2652890]

RadioImmunoPrecipitation Assay (RIPA) Buffer, 10X [orb2652891]

10X RIPA Lysis Buffer [orb420113]

Protocol Information