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> 14-3-3, sigma (SFN, Stratifin, HGNC:10773) (BSA & Azide Free) (MaxLight 490)

14-3-3, sigma (SFN, Stratifin, HGNC:10773) (BSA & Azide Free) (MaxLight 490)

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Référence 0014-07A-ML490-100ul

Conditionnement : 100ul

Marque : US Biological

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0014-07A-ML490 14-3-3, sigma (SFN, Stratifin, HGNC:10773) (BSA & Azide Free) (MaxLight 490)

Clone Type
Monoclonal
Host
mouse
Source
human
Isotype
IgG1,k
Grade
Affinity Purified
Applications
IF IHC IP WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

14-3-3 proteins are 30kD polypeptides with nine closely related members in mammals. They are also found in plants and fungi. 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins which bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. 14-3-3 proteins form homo- and heterodimeric cup-like structures that bind to discrete phosphoserine-containing motifs. In some instances, 14-3-3 proteins appear to export their binding partners from the nucleus to the cytoplasm in a phosphorylation and Crm1-dependent manner. 14-3-3 sigma shares cyclin-Cdk2 binding motifs with different cell cycle regulators, including p107, p130, p21Cip1, p27Kip1 and p57Kip2 and is associated with cyclin-Cdk complexes in vitro and in vivo. Expression of 14-3-3 sigma is higher in normal epithelial cells.

Applications:
Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Formalin/paraffin): Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
HT29 cells, Skin

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. 

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1,k|Clone No: 1.N.6|Host: mouse|Source: human|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.|Purity: Purified by Protein G affinity chromatography from ascites.|Immunogen: Recombinant protein corresponding to human 14-3-3 sigma protein. Cellular Localization: Cytoplasmic.|Specificity: Recognizes human 14-3-3 sigma protein (26-30kD). Does not crossreact with other isoforms of 14-3-3.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Recombinant protein corresponding to human 14-3-3 sigma protein. Cellular Localization: Cytoplasmic.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Purity
Purified by Protein G affinity chromatography from ascites.
Specificity
Recognizes human 14-3-3 sigma protein (26-30kD). Does not crossreact with other isoforms of 14-3-3.
References
1. Laronga, C., et al., J. Biol. Chem. 275: 23,106-23,112 (2000). 2. Villaret, D.B., et al., Laryngoscope 110: 374-381 (2000). 3. Hermeking, H., et al., Mol. Cell 1: 3-11 (1997).