2-Aminoethyl Methanethiosulfonate, Hydrochloride (MTSEA-Chloride)
Référence MBS6048181-25mg
Conditionnement : 25mg
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Methanesulfonothioic Acid S-(2-Aminoethyl) Ester Hydrochloride; MTSEA-Chloride
2-Aminoethyl Methanethiosulfonate, Hydrochloride (MTSEA-Chloride)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6048181Reacts specifically and rapidly with thiols to form mixed disulfides. Used to probe the structures of the ACh receptor channel of the GABA receptor channel and of lactose permease. Useful for mapping the pore-lining regions of the ryanodine receptor. More details
Product Overview
Online Datasheet in PDFProduct Name
2-Aminoethyl Methanethiosulfonate, Hydrochloride, Biochemical
Full Product Name
2-Aminoethyl Methanethiosulfonate, Hydrochloride (MTSEA-Chloride)
Preparation and Storage
Some methanethiosulfonates are hygroscopic and all hydrolyze in water, over a period of time, particularly in the presence of nucleophiles. They should be stored in a desiccator at minus 20 degree C and warmed up to room temperature before opening the vial. For maximum results, solutions should be made up immediately prior to use even though solutions in distilled water appear to be stable for hours at 4 degree C.
At pH 7.5 and room temperature, MTSEA hydrolyzes with a half-life of about 15 minutes, MTSET hydrolyzes with a half-life of 10 minutes, and MTSES hydrolyzes with a half-life of 20 minutes. Routinely, one can use 2.5mM MTSEA, 1mM MTSET, or 10mM MTSES, applied for 1 to 5 minutes. (MTSET is 2.5 times as reactive with small sulfhydryl compounds as is MTSEA, and 10 times as reactive as MTSES). (Stauffer and Karlin, 1994; Karlin, personal communication).
For application to Xenopus Oocytes, dissolve the compounds in 115mM NaCl, 2.5mM KCl, 1.8mM MgCl2, 10mM Hepes, pH 7.5 with NaOH. MTS reagents decompose in buffer very quickly.
•DMSO is a good solvent for the MTS reagents which are not water soluble (i.e. the non-charged MTS reagents).
At pH 7.5 and room temperature, MTSEA hydrolyzes with a half-life of about 15 minutes, MTSET hydrolyzes with a half-life of 10 minutes, and MTSES hydrolyzes with a half-life of 20 minutes. Routinely, one can use 2.5mM MTSEA, 1mM MTSET, or 10mM MTSES, applied for 1 to 5 minutes. (MTSET is 2.5 times as reactive with small sulfhydryl compounds as is MTSEA, and 10 times as reactive as MTSES). (Stauffer and Karlin, 1994; Karlin, personal communication).
For application to Xenopus Oocytes, dissolve the compounds in 115mM NaCl, 2.5mM KCl, 1.8mM MgCl2, 10mM Hepes, pH 7.5 with NaOH. MTS reagents decompose in buffer very quickly.
•DMSO is a good solvent for the MTS reagents which are not water soluble (i.e. the non-charged MTS reagents).
Molecular Formula
C3H9NO2S2• HCl
Melting Point
145-147 degree C
Solubility
DMSO, Warm Ethanol, Methanol, Water,
Product Categories/Family for 2-Aminoethyl Methanethiosulfonate, Hydrochloride biochemical
Biochemicals; Biochem-Maleimide; MTS & Sulfhydryl
Other Notes
Small volumes of 2-Aminoethyl Methanethiosulfonate, Hydrochloride biochemical vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for 2-Aminoethyl Methanethiosulfonate, Hydrochloride purchase
MBS6048181 is a biochemical targeting 2-Aminoethyl Methanethiosulfonate, Hydrochloride. Also known as 2-Aminoethyl Methanethiosulfonate, Hydrochloride (MTSEA-Chloride).