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2-Iodoacetamide [144-48-9]

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Référence NB-64-45250-1mL

Conditionnement : 1mLx10mM(inDMSO)

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Marque : Neo Biotech

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2-Iodoacetamide

(Synonyms: Iodoacetamide) Copy Product Info

Synonyms: Iodoacetamide

Catalog No. T8897 Copy Product Info

Purity: 99.95%

Iodoacetamide, an alkylating agent, is commonly utilized for cysteine alkylation in proteomics sample preparation.

Cas No. 144-48-9

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.95%

Appearance:Solid

Color:White to Yellow

Product Introduction

2-Iodoacetamide AI Summary

2-Iodoacetamide exhibits a broad spectrum of bioactivities and notable inhibitory capabilities. It is effective in inactivating rat muscle adenylate kinase and inhibiting adenylate kinase II, influencing enzyme inactivation half-time and rate. The compound also shows potent inhibition of horse liver alcohol dehydrogenase with a Ki value of 1905460.72 nM, and exhibits a logP value of 0.15. Furthermore, 2-Iodoacetamide significantly inhibits Aryl Hydrocarbon Activation (AHAT) by 99.0% at a 1 mM concentration using compound 8 and compound 10 as substrates. In addition, it inhibits thymidylate synthetase with a rate constant (k2) of 7.0 M^-1 at pH 7 and 24°C, and demonstrates potent inhibition of HIV YU2 gp120 binding to CD4 with an IC50 value of 33.3 nM. The compound also activates TRPA1 with an EC50 value of 357000.0 nM. In enzyme assays, 2-Iodoacetamide shows inhibitory activity against HSD17B4, RECQ1, JMJD2E, Tyrosyl-DNA Phosphodiesterase (TDP1), Cytochrome P450 2C19, Aldehyde Dehydrogenase 1 (ALDH1A1), and others, indicating extensive modulation across various biological pathways. It also displays significant activity in assays involving Lamin A Splicing, Histone Lysine Methyltransferases such as G9a, and the Regulator of G Protein Signaling (RGS) 4, among others. Moreover, 2-Iodoacetamide inhibits Pantoea ananatis lycopene cyclase, leaving 87.0% residual enzyme activity at 0.5 mM concentration and exhibits cysteine reactivity with a determined second-order rate constant of 25.0 /M/s assessed by 1H NMR. The compound also binds to human plasma coagulation factor XIIIa with a rate constant of 0.8 x 10^4/M/s and demonstrates a significant rate constant ratio to Glutathione. Overall, 2-Iodoacetamide’s diverse bioactivities and broad inhibitory profile underscore its potential as a multi-target therapeutic agent..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Iodoacetamide, an alkylating agent, is commonly utilized for cysteine alkylation in proteomics sample preparation.
Synonyms	Iodoacetamide

Chemical Properties

Molecular Weight	184.96
Formula	C₂H₄INO
Cas No.	144-48-9
Smiles	NC(=O)CI
Relative Density.	2.1103 g/cm3 (Estimated)

Storage & Solubility Information

Storage

Keep away from direct sunlight,Store at low temperature Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (270.33 mM), Sonication is recommended.

H2O: 50 mg/mL (270.33 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (10.81 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO/H2O

	1mg	5mg	10mg	50mg
1 mM	5.4066 mL	27.0329 mL	54.0657 mL	270.3287 mL
5 mM	1.0813 mL	5.4066 mL	10.8131 mL	54.0657 mL
10 mM	0.5407 mL	2.7033 mL	5.4066 mL	27.0329 mL
20 mM	0.2703 mL	1.3516 mL	2.7033 mL	13.5164 mL
50 mM	0.1081 mL	0.5407 mL	1.0813 mL	5.4066 mL
100 mM	0.0541 mL	0.2703 mL	0.5407 mL	2.7033 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

peptidasescysteinealkylationalkylating2-Iodoacetamide2Iodoacetamide2 Iodoacetamide

Related Tags: 2-Iodoacetamide chemical structure | 2-Iodoacetamide formula | 2-Iodoacetamide molecular weight

2-Iodoacetamide [144-48-9]

Référence NB-64-45250-1mL

Contactez votre distributeur local :

Marque : Neo Biotech

Contactez votre distributeur local :

2-Iodoacetamide

Product Introduction

Calculator

In Vivo Formulation Calculator (Clear solution)

Dose Conversion

Keywords

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