25-hydroxy vitamin D3 (25 HVD3) BioAssay™ ELISA Kit (Guinea pig)

Sample Type:
Serum

Intended Use:
For the quantitative determination of endogenic Guinea pig 25-hydroxy vitamin D3 (25(OH)D3/25 HVD3) concentrations in serum.

Sensitivity:
5ug/L

Range:
20-100ug/L.

Specificity:
This assay has high sensitivity and excellent specificity for detection of Guinea pig 25HVD3. No significant cross-reactivity or interference between Guinea pig 25HVD3 and analogues was observed.

Test Principle:
This BioAssay™ employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated 25HVD3 Hapten. The competitive inhibition reaction is launched between with HRP-conjugated 25HVD3 Hapten and 25HVD3 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of 25HVD3 in the sample. The color development is stopped and the intensity of the color is measured.

Kit Components:
Assay plate, 1x96 wells
Standard, 5x0.5ml
High Value Control, 1x0.5ml
Middle Value Control, 1x0.5ml
Sample Diluent, 1x50ml
HRP-conjugate, 1x7ml
Wash Buffer (20X), 1x30ml
Substrate A, 1x7ml
Substrate B, 1x7ml
Stop Solution, 1x7ml

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.