Orb1146751-48 | 4-HNE(4-Hydroxynonenal) ELISA Kit Biovalley

4-HNE(4-Hydroxynonenal) ELISA Kit

Catalog Number	orb1146751
Category	Assays and Kits
Description	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 4-HNE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.
Reactivity	All
Range	0.63-40 ng/mL
Sensitivity	0.38 ng/mL
Protein Length	2h
Alternative names	4-HNE; HNE; 4-hydroxy-2-nonenal; 4-Hydroxynonenal
Note	For research use only
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Expiration Date	Please enquire.