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> 4E Binding Protein 1 (Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)

4E Binding Protein 1 (Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)

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Référence 0004-18-100ul

Conditionnement : 100ul

Marque : US Biological

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0004-18 4E Binding Protein 1 (Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)

Clone Type
Polyclonal
Host
rabbit
Source
human
Swiss Prot
Q13541
Isotype
IgG
Grade
Affinity Purified
Applications
IP WB
Crossreactivity
Hu Mk Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

4E-BP1 (also known as PHAS-1) normally binds eIF4E, inhibiting cap-dependent translation. Hyperphosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation (1). The PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 (2,3). 4E-BP1 is phosphorylated in vivo on multiple residues (4); phosphorylation by FRAP/mTOR on Thr37 and Thr46 of human 4E-BP1 may prime it for subsequent phosphorylation at sites including Ser65 and Thr70 (5).

Applications:
Suitable for use in Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: human|Concentration: Not Determined|Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.|Purity: Purified by protein A and peptide affinity chromatography.|Immunogen: Synthetic peptide corresponding to residues surrounding Ser112 of human 4E-BP1. MW of Antigen: 15-20kD.|Specificity: Recognizes endogenous levels of total human 4E-BP1 independent of phosphorylation. Species Crossreactivity: mouse, rat and monkey.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Synthetic peptide corresponding to residues surrounding Ser112 of human 4E-BP1. MW of Antigen: 15-20kD.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity
Purified by protein A and peptide affinity chromatography.
Specificity
Recognizes endogenous levels of total human 4E-BP1 independent of phosphorylation. Species Crossreactivity: mouse, rat and monkey.
References
(1) Pause, A. et al. (1994) Nature 371, 762–767. (2) Brunn, G.J. et al. (1997) Science 277, 99–101. (3) Gingras, A.C. et al. (1998) Genes Dev. 12, 502–513. (4) Fadden, P. et al. (1997) J. Biol. Chem. 272, 10240–10247. (5) Gingras, A.C. et al. (1999) Genes Dev. 13, 1422–1437.