The 5-Hydroxytryptamine (5-HT) BioAssay™ ELISA Kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of 5-HT in serum and plasma.

Detection Range:
11.11-900ng/ml

Sensitivity:
3.97ng/ml

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components:
*023037A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use
*023037B: Standard, 2x1 vial
023037C: Standard Diluent, 1x20ml
*023037D: Detection Reagent A (green), 1x1vial
*023037E: Detection Reagent B (red), 1x120ul
023037F: Assay Diluent A, 1x12ml
023037G: Assay Diluent B, 1x12ml
023037H: TMB Substrate, 1x9ml
*023037J: Positive Control, 1x1vial, xxng/ml (Lyophilized)
023037L: Wash Buffer, 30X, 1x20ml
023037M: Reagent Diluent, 1x300ul

Storage and Stability:
Store *023037A, *023037B, *023037D, *023037E and *023037J at -20°C. After reconstitution of *023037J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary:
1. Prepare all reagents, samples and standards;
2. Add 50ul standard or sample to each well.
3. Add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C.
8. Add 50ul Stop Solution. Read at 450nm immediately.