Acridine Orange Hydrochloride Hydrate (CI 46005) [65-61-2]

Acridine Orange Hydrochloride is the hydrocloride salt of Acridine Orange Stain, which is a fluorescent nucleic acid binding dye that interacts with both DNA and RNA. When bound to DNA, it emits green fluorescence (Em=525 nm) and when bound to RNA, it emits red fluorescence (Em=~650 nm). Acridine Orange Stain has significantly distinguished cell death by apoptosis and necroptosis in human oligodendrocytes and mouse models.

Synonyms:
3,6-Bis(dimethylamino)acridine hydrochloride; CI 46005

CAS No:
65-61-2

Molecular Formula:
C17H19N3·HCl·xH2O

Molecular Weight:
301.82

Appearance:
Dark orange or brick red powder

Purity:
≥99% (HPLC)

Melting Point:
284-287°C (lit.)

UV Ratios (10mg/L in water):
489-495 nm = 1.160
265-271 nm = 1.160
227-233 nm = 0.464

Extinction Co-Efficient (water):
E (489-495 nm) : ≥35000
E (265-271 nm) : ≥35000
E (227-233 nm) : ≥14000

Water (KF):
≤20%

Solubility:
Water: 0.1%, clear, orange to red solution

Storage and Stability:
May be stored at RT. Light sensitive.