ACTB (Actin, Cytoplasmic 1, Beta-actin) (Biotin)

In vertebrates 3 main groups of actin isoforms, A, B and G have been identified. The A-actins are found in muscle tissues and are a major constituent of the contractile apparatus. The B and G-actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. B-actins are highly conserved proteins that are involved in cell motility, structure and integrity. B-actins are cytoplasmic proteins.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100,000
Western Blot: 1:2000-1:10,000
Immunohistochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.