Adenovirus Hexon Antigen Capture BioAssay™ ELISA Kit

The Adenovirus Hexon Antigen Capture BioAssay™ ELISA Kit is a qualitative procedure for the determination of adenovirus hexon antigen in a variety of sample matrices, including feces.

Acute diarrheal disease in young children is a major cause of morbidity worldwide and is a leading cause of mortality in developing countries
.Research has shown that enteric adenoviruses, primarily Ad40 and Ad41, are a leading cause of diarrhea in many of these children, second only to the rotaviruses. These viral pathogens have been isolated throughout the world, and can cause diarrhea in children year round. Infections are most frequently seen in children under two years of age, but have been found in patients of all ages. Further studies indicate that adenoviruses are associated with 4 - 15% of all hospitalized cases of viral gastroenteritis.

Many laboratories use electron microscopy (EM) to detect viruses associated with gastroenteritis. Other techniques include direct genome profiling and nucleic acid hybridization, neither of which is rapid or specific. Alternatively, ELISA tests using Adenovirus-specific antibodies have been shown to be a sensitive, specific, and rapid methods for the detection of enteric adenoviruses.

Test Principle:
The assay is a double antibody (sandwich) ELISA that utilizes a monoclonal anti-adenovirus antibody to capture the antigen from the sample. Following sample incubation, a biotinylated detection antibody is added and this step is followed by HRP Streptavidin. The presence of hexon antigen is visualized by the addition of an HRP substrate followed by the addition of a stop reagent. The development of color within the well is indicative of the presence of adenovirus hexon antigen in the sample.

For quantitative purposes, use Adenovirus Antigen Calibration Kit (Catalog Number 045731) which can be used in conjunction with this product to generate a standard curve for quantitative measurement.

The kit has been tested against a variety of adenovirus subtypes for sensitivity and potentially interfering viruses and bacteria for specificity.

Kit Components:
1. Adenovirus Antigen Capture Plate, 2x96Tests
2. Sample Preparation Reagent, 1x12ml
3. Adenovirus Positive Control, 1x1ml
4. Adenovirus Negative Control, 2x1.5ml
5. Wash Buffer, 2x50ml (20X)
6. Adenovirus Detection Antibody, Biotin-labeled, 1x22ml
7. Streptavidin-HRP, 1x22ml
8. Chromagen Solution, 1x22ml
9. Stop Solution, 1x22ml
10. Sample Dilution Tray, 2 ea.

Optional Components:
045731--Adenovirus Antigen Calibration Kit

Storage and Stability:
Store all kit components at 2-8°C. Crystal formation may occur in the wash buffer concentrate during prolonged storage at 2-8°C. The crystals can be re-dissolved by swirling the bottle in warm tap water. Stable for 6 months after receipt.