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AM1-43

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Référence 70024

Conditionnement : 1MG

Marque : Biotium

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AM1-43 (Equivalent to FM®1-43FX)

AM1-43 (equivalent to FM®1-43FX) is a fixable, activity-dependent fluorescent nerve terminal probe. AM1-43 functions similarly similarly to SynaptoGreen C4 (catalog # 70020), but with the additional property of being fixable.

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AM1-43
AM1-43 (Equivalent to FM®1-43FX) - Image 2Mouse cochlear hair cell neurons labeled with AM1-43. Image courtesy of Dr. David Corey, Harvard Medical School.
AM1-43 (Equivalent to FM®1-43FX) - Image 3The apical half of a single mouse vibrissal follicle, labeled with AM1-43. Image courtesy of Dr. David Corey, Harvard Medical School.
  1. AM1-43
  2. AM1-43 (Equivalent to FM®1-43FX) - Image 2
  3. AM1-43 (Equivalent to FM®1-43FX) - Image 3
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Product Description

AM1-43 (equivalent to FM®1-43FX) is a fixable, activity-dependent fluorescent nerve terminal probe. AM1-43 functions similarly to SynaptoGreen™ C4 (70020), but with the additional property of being fixable. Thus, AM1-43 is a useful tool for synapse studies where subsequent fluorescent immunochemistry is desired. AM1-43 and FM®1-43FX have identical excitation and emission wavelengths. Also see AM1-44 (catalog no. 70038), which is similar to AM1-43, but has longer lipophilic tails, resulting in better fixability.

  • Ex/Em: 510/625 nm (in MeOH); 480/598 nm (in membranes)
  • Red solid soluble in water
  • C29H49Cl3N4
  • MW: 559.5


Nerve Terminal Dyes and Kits

ProductCatalog No.Features
SynaptoGreen™ Dyes
(Ex/Em ~480/660 nm in membranes)		70020... 70053View full list of SynaptoGreen™ Dyes
SynaptoRed Dyes™
(Ex/Em ~510/750 nm in membranes)		70021... 70050View full list of SynaptoRed™ Dyes
Background Reducers
ADVASEP-770029Sulfonated cyclodextrin that aids in removal of free dye during washes
SCAS70037Quenches extracellular fluorescences with fewer wash steps than ADVASEP-7
Sulforhodamine 10180101Red fluorescent dye that quenches extracellular fluorescence of SynaptoGreen™ dyes
Nerve Terminal Staining Kits
Nerve Terminal Staining Kit I70030Includes SynaptoGreen™ C4 and ADVASEP-7
Nerve Terminal Staining Kit II (A)70031Includes AM1-43 and ADVASEP-7
Nerve Terminal Staining Kit II (B)70031-1Includes AM1-43 and SCAS
Nerve Terminal Staining Kit III70032Includes SynaptoGreen™ C4 and Sulforhodamine 101
Nerve Terminal Staining Kit V70034Includes SynaptoRed™ C2 and ADVASEP-7
m is the number of carbons in the lipophilic tail and n is the number of double bonds linking the two aromatic rings in the dye.
**The positively-charged end of SynaptoRed C2M is a trimethylammonium group.
FM is a registered trademark of Thermo Fisher Scientific.
Technology
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Membrane Potential & Nerve Terminal Dyes
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Neuronal Probes and Tools

Product Attributes

Size
1 mg
Probe cellular localization
Membrane/vesicular
For live or fixed cells
For live/intact cells
Assay type/options
Real-time imaging
Fixation options
Fix after staining (formaldehyde), Permeabilize after staining
Colors
Green
Excitation/Emission
480/598 nm (in membranes)

Documents, Protocols, SDS and COA

COA
Request the Certificate of Analysis
References

1. PLoS One, 5(8), e12386.

2. Anat Cell Biol 44(4), 274 (2011).

Citations

1. PLoS One, 5(8), e12386.

2. Anat Cell Biol 44(4), 274 (2011).

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