Conditionnement : 1mLx10mM(inDMSO)
Téléphone : +1 850 650 7790
Synonyms: D-(-)-α-Aminobenzylpenicillin, Ampicillin acid, Aminobenzylpenicillin, Amcill
| Description | Ampicillin (Aminobenzylpenicillin) is a semi-synthetic penicillin belonging to the β-lactam group of antibiotics. Ampicillin has bactericidal activity against a wide range of Gram-positive and Gram-negative bacteria. |
| Targets & IC50 | enzyme transpeptidase:1.8 µg/mL, transporter:9.9 mM (Ki), carnitine/carnitine antiport:7.6 mM, HEK293 cells:> 100 μM (CC50) |
| In vitro | METHODS: E. coli was treated with Ampicillin (0.1-10 μG/mL) for 6 h. Growth was measured using a Fisher Electrophotometer. RESULTS: No differences in E. coli growth were observed at concentrations of 0, 0.1, and 1.0 μG. 2.5, 5, and 10 μG concentrations of Ampicillin inhibited E. coli growth. [1] METHODS: Pharyngeal cancer cells Detroit-562 were treated with Ampicillin (10-100 μM) for 72 h. Cell viability was measured by MTT. RESULTS: The Ampicillin-treated groups at concentrations of 10, 25 and 50 μM showed higher cell viability than the control cells, with values of 112%, 107% and 106%, respectively. At concentrations of 75 and 100 μM, there was a slight but not significant decrease in cell viability with values of approximately 95% and 94%, respectively. [2] |
| In vivo | METHODS: To test the neuroprotective mechanism in a mouse model of transient total forebrain ischemia, Ampicillin (200 mg/kg) was injected intraperitoneally into C57BL/6 mice once a day for five days, and forebrain ischemia was induced 24 h after the last injection. RESULTS: Pretreatment with Ampicillin significantly attenuated neuronal damage in the CA1 subregion of the hippocampus, and Ampicillin may provide neuroprotection against cerebral ischemia/reperfusion injury by inducing GLT-1 protein and inhibiting MMP activity in the hippocampus of mice. [3] METHODS: To study the in vivo activity, Ampicillin (10-100 mg/kg) was administered by gavage to C57BL/6 mice once daily for two days. RESULTS: Exposure of mice to Ampicillin induced anxiety and colitis, and increased Aspergillus flora in the gut microbiota. [4] |
| Synonyms | D-(-)-α-Aminobenzylpenicillin, Ampicillin acid, Aminobenzylpenicillin, Amcill |
| Molecular Weight | 349.4 |
| Formula | C16H19N3O4S |
| Cas No. | 69-53-4 |
| Smiles | 10[C@@]1(NC(=O)[C@H](N)c2ccccc2)C(=O)N2[C@@H](C(O)=O)C(C)(C)S[C@]1210 |
| Relative Density. | 1.2794 g/cm3 (Estimated) |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | ||||||||||||||||||||||||||||||||||||||||
| Solubility Information | DMSO: 240 mg/mL (686.89 mM), Sonication is recommended.  0.1 M NaOH: 25 mg/mL (71.55 mM), Sonication is recommended. | ||||||||||||||||||||||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||||||||||||||||||||||
0.1 M NaOH/DMSO
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | |||||||||||||||||||||||||||||||||||||||||
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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