Amyloid beta A4 (Phospho-Thr743/668) Colorimetric Cell-Based ELISA Kit
Référence EKC1937
Conditionnement : 1kit,containingtwo96-wellplatesandallnecessaryreagents
Marque : Boster Bio
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Téléphone : +1 850 650 7790
Product Info Summary
| SKU: | EKC1937 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
| Sample Types: | Cell lines |
Product Name
Amyloid beta A4 (Phospho-Thr743/668) Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC1937
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The Amyloid beta A4 (Phospho-Thr743/668) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Amyloid beta A4 (Phospho-Thr743/668) protein expression profile in cells. The kit can be used for measuring the relative amounts of Amyloid beta A4 (Phospho-Thr743/668) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Amyloid beta A4 (Phospho-Thr743/668).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
Amyloid beta A4 (Phospho-Thr743/668) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1937)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1937 is reactive to APP in Human, Mouse, Rat samples
Validated Sample Types
Cell lines
Application Guarantee
EKC1937 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 30 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 60 ml (15x) | Clear |
| 100x Anti-Amyloid beta A4 Phospho-Thr743/668 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-Amyloid beta A4 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)