This human monoclonal antibody clone ANDV44 was generated as part of a panel using a B cell hybridoma method from an individual previously infected with ANDV⁸.

This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multistep process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

This antibody may be stored sterile as received at 28°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ 70°C. Avoid Repeated Freeze Thaw Cycles.

Standard Overnight on Blue Ice.

ELISA
N: ANDV44 shows neutralizing activity for ANDV, authentic Sin Nombre virus (SNV), crossreactivity to SNV Gn and Gc proteins, and potent neutralizing activity to authentic OWH Hantaan virus strain 76–118.

Clone ANDV44 competes for binding to a distinct site on the ANDV surface glycoprotein spike, a tetrameric complex composed of Gn and Gc envelope proteins⁸. Furthermore, antibody ANDV44 recognizes antigenic site C1 but does not show reactivity to soluble Gc or Gn proteins and may bind to a target site only present in the quaternary spike structure.

Hantavirus or Andes Virus is an enveloped, negativesensed, singlestranded RNA virus in the bunyavirus family¹. “New World” hantaviruses (NWH) are found in the Americas and may cause hantavirus pulmonary syndrome (HPS)². “Old World” hantaviruses (OWH) are found mainly in Europe and Asia and may cause hemorrhagic fever with renal syndrome. Each hantavirus serotype has a specific rodent host species, and infection is spread primarily by aerosolized feces, urine, or saliva, with the exception of Andes Virus (ANDV) which is also capable of humantohuman transmission^3,4.

ANDV, a NWH found in South America⁵, has a high fatality rate of up to 40%⁶. Clinical research shows that high titers of neutralizing antibodies in patient serum correlate with increased survival⁷, and monoclonal antibodies (mAbs), including ANDV44, isolated from HPS survivors protect Syrian hamsters from ANDV postinfection⁶⁸.

1. Centers for Disease Control and Prevention, National Center for Emerging and Zoonotic Infectious Diseases (NCEZID), Division of HighConsequence Pathogens and Pathology (DHCPP),
2. Centers for Disease Control and Prevention, National Center for Emerging and Zoonotic Infectious Diseases (NCEZID), Division of HighConsequence Pathogens and Pathology (DHCPP), Link
3. Padula PJ, Edelstein A, Miguel SD, et al. Virology. 241:323–330. 1998.
4. Martinez VP, Bellomo C, San Juan J, et al. Emerg Infect Dis. 11:1848–1853. 2005.
5. Centers for Disease Control and Prevention, National Center for Emerging and Zoonotic Infectious Diseases (NCEZID), Division of HighConsequence Pathogens and Pathology (DHCPP), https://www.cdc.gov/hantavirus/resources/andesvirus.html
6. Williamson BN, Prescott J, Garrido JL, et al. Emerg Infect Dis. 27(10):27072710. 2021.
7. Bharadwaj M, Nofchissey R, Goade D, et al. J Infect Dis. 182: 43–48. 2000.
8. Engdahl TB, Kuzmina NA, Ronk AJ, et al. Cell Rep. 35(5):109086. 2021.
9. Garrido, JL, et al. (2018) Sci Transl Med.10(468):eaat6420.