Anti-CD10 Antibody
Référence MBS177054-0,1mg-APC
Conditionnement : 0.1mg(APC)
Marque : MyBiosource
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Téléphone : +1 850 650 7790
Anti-CD10 Picoband antibody, MBS177054-1.JPG
IHC(P): Mouse Kidney Tissue
IHC(P): Mouse Kidney Tissue
Anti-CD10 Picoband antibody, MBS177054-2.JPG
IHC(P): Human B lymphocyte Cancer Tissue
IHC(P): Human B lymphocyte Cancer Tissue
Anti-CD10 Picoband antibody, MBS177054-3.JPG
IHC(P): Mouse Spleen Tissue
IHC(P): Mouse Spleen Tissue
Anti-CD10 Picoband antibody, MBS177054-4.JPG
IHC(P): Rat Spleen Tissue
IHC(P): Rat Spleen Tissue
Anti-CD10 Picoband antibody, MBS177054-5.jpg
All lanes: Anti-CD10(MBS177054) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 40ug
Lane 2: Rat Brain Tissue Lysate at 40ug
Lane 3: Rat Liver Tissue Lysate at 40ug
Lane 4: Human Placenta Tissue Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: JURKAT Whole Cell Lysate at 40ug
Lane 7: RAJI Whole Cell Lysate at 40ug
Lane 8: 293T Whole Cell Lysate at 40ug
Predicted bind size: 86KD
Observed bind size: 100KD
All lanes: Anti-CD10(MBS177054) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 40ug
Lane 2: Rat Brain Tissue Lysate at 40ug
Lane 3: Rat Liver Tissue Lysate at 40ug
Lane 4: Human Placenta Tissue Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: JURKAT Whole Cell Lysate at 40ug
Lane 7: RAJI Whole Cell Lysate at 40ug
Lane 8: 293T Whole Cell Lysate at 40ug
Predicted bind size: 86KD
Observed bind size: 100KD
Applications: Western Blot
Sample: Rat PFC
Detection: Protein Simple, Simple Western size-based assay
"We selected this product as a polyclonal ab for our Tg Rat colony. We are interested in adding enzymatic catabolism/clearance of amyloid beta isoforms into our body of research. We purchased this product because it was cheap and we are looking at some 'proof-of-concept' and didn't need remarkable specificity. We still have much to investigate."
Sample: Rat PFC
Detection: Protein Simple, Simple Western size-based assay
"We selected this product as a polyclonal ab for our Tg Rat colony. We are interested in adding enzymatic catabolism/clearance of amyloid beta isoforms into our body of research. We purchased this product because it was cheap and we are looking at some 'proof-of-concept' and didn't need remarkable specificity. We still have much to investigate."
CD10 (MME) Antibody – Rabbit Polyclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177054Description: Rabbit IgG polyclonal antibody for Neprilysin(MME) detection. Tested with WB, IHC-P in Human, Mouse, Rat.Background: CD10, also known as membrane metallo-endopeptidase, neutral endopeptidase(NEP), Neprilysin, or common acute lymphoblastic… More details
Product Overview
Online Datasheet in PDFProduct Name
CD10 (MME), Polyclonal Antibody
Full Product Name
Anti-CD10 Antibody
Product Gene Name
anti-MME antibody
Product Synonym Names
Neprilysin; membrane metallo-endopeptidase; Atriopeptidase antibody; CALLA antibody; CD10 antibody; CD10 antigen antibody; Common acute lymphocytic leukemia antigen antibody; DKFZp686O16152 antibody; EC 3.4.24.11 antibody; Enkephalinase antibody; EPN antibody; gp100 antibody; Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) antibody; Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibody; Membrane metallo endopeptidase antibody; Membrane metallo endopeptidase variant 1 antibody; Membrane metallo endopeptidase variant 2 antibody; Membrane metalloendopeptidase antibody; Membrane metalloendopeptidase neutral endopeptidase enkephalinase antibody; Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 antibody; Membrane metalloendopeptidase variant 1 antibody; Membrane metalloendopeptidase variant 2 antibody; MGC126681 antibody; MGC126707 antibody; MME antibody; NEP antibody; NEP_HUMAN antibody; Neprilysin antibody; Neutral endopeptidase 24.11 antibody; Neutral endopeptidase antibody; SFE antibody; Skin fibroblast elastase antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
85,514 Da
OMIM
120520
Immunogen
E Coli-derived human CD10 recombinant protein (Position: Y52-W750). Human CD10 shares 94% amino acid (aa) sequences identity with both mouse and rat CD10.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-MME antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Testing Data of anti-MME antibody
Anti-CD10 Picoband antibody, MBS177054-5.jpg
All lanes: Anti-CD10(MBS177054) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 40ug
Lane 2: Rat Brain Tissue Lysate at 40ug
Lane 3: Rat Liver Tissue Lysate at 40ug
Lane 4: Human Placenta Tissue Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: JURKAT Whole Cell Lysate at 40ug
Lane 7: RAJI Whole Cell Lysate at 40ug
Lane 8: 293T Whole Cell Lysate at 40ug
Predicted bind size: 86KD
Observed bind size: 100KD
All lanes: Anti-CD10(MBS177054) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 40ug
Lane 2: Rat Brain Tissue Lysate at 40ug
Lane 3: Rat Liver Tissue Lysate at 40ug
Lane 4: Human Placenta Tissue Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: JURKAT Whole Cell Lysate at 40ug
Lane 7: RAJI Whole Cell Lysate at 40ug
Lane 8: 293T Whole Cell Lysate at 40ug
Predicted bind size: 86KD
Observed bind size: 100KD
Western Blot (WB) of anti-MME antibody
Applications: Western Blot
Sample: Rat PFC
Detection: Protein Simple, Simple Western size-based assay
"We selected this product as a polyclonal ab for our Tg Rat colony. We are interested in adding enzymatic catabolism/clearance of amyloid beta isoforms into our body of research. We purchased this product because it was cheap and we are looking at some 'proof-of-concept' and didn't need remarkable specificity. We still have much to investigate."
Sample: Rat PFC
Detection: Protein Simple, Simple Western size-based assay
"We selected this product as a polyclonal ab for our Tg Rat colony. We are interested in adding enzymatic catabolism/clearance of amyloid beta isoforms into our body of research. We purchased this product because it was cheap and we are looking at some 'proof-of-concept' and didn't need remarkable specificity. We still have much to investigate."
Other Notes
Small volumes of anti-MME antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for MME purchase
MBS177054 is an antibody targeting MME. Also known as Anti-CD10 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.
Additional Details
NCBI/Uniprot data below describe general gene information for MME. It may not necessarily be applicable to this product.
NCBI Official Full Name
Neprilysin
NCBI Official Synonym Full Names
membrane metallo-endopeptidase
NCBI Official Symbol
MME
NCBI Official Synonym Symbols
NEP; SFE; CD10; CALLA
UniProt Protein Name
Neprilysin
UniProt Synonym Protein Names
Atriopeptidase; Common acute lymphocytic leukemia antigen; CALLA; Enkephalinase; Neutral endopeptidase 24.11; NEP; Neutral endopeptidase; Skin fibroblast elastase; SFE; CD_antigen: CD10
Protein Family
Neprilysin
UniProt Gene Name
MME
UniProt Synonym Gene Names
EPN; CALLA; NEP; Neutral endopeptidase; SFE
UniProt Entry Name
NEP_HUMAN
NCBI Protein Information
neprilysin; enkephalinase; atriopeptidase; neprilysin-390; neprilysin-411; skin fibroblast elastase; neutral endopeptidase 24.11; membrane metallo-endopeptidase variant 1; membrane metallo-endopeptidase variant 2; common acute lymphocytic leukemia antigen; membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase); membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10)
NCBI Summary for MME
This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. [provided by RefSeq, Jul 2008]
Show more UniProt Comments for MME
CD10: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV- inducible elastase activity toward skin preelastic and elastic fibers. Inhibited in a dose dependent manner by opiorphin. Belongs to the peptidase M13 family.
Protein type: Protease; Membrane protein, integral; EC 3.4.24.11
Chromosomal Location of Human Ortholog: 3q25.2
Cellular Component: synaptic vesicle; focal adhesion; axon; integral to plasma membrane; cytoplasm; dendrite; integral to membrane; plasma membrane; synapse; brush border
Molecular Function: protein binding; zinc ion binding; metalloendopeptidase activity; endopeptidase activity; exopeptidase activity; peptide binding
Biological Process: peptide metabolic process; cellular protein metabolic process; beta-amyloid metabolic process; creatinine metabolic process; proteolysis; sensory perception of pain; angiotensin maturation; kidney development
Disease: Membranous Nephropathy, Susceptibility To
Show more Protein type: Protease; Membrane protein, integral; EC 3.4.24.11
Chromosomal Location of Human Ortholog: 3q25.2
Cellular Component: synaptic vesicle; focal adhesion; axon; integral to plasma membrane; cytoplasm; dendrite; integral to membrane; plasma membrane; synapse; brush border
Molecular Function: protein binding; zinc ion binding; metalloendopeptidase activity; endopeptidase activity; exopeptidase activity; peptide binding
Biological Process: peptide metabolic process; cellular protein metabolic process; beta-amyloid metabolic process; creatinine metabolic process; proteolysis; sensory perception of pain; angiotensin maturation; kidney development
Disease: Membranous Nephropathy, Susceptibility To
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with anti-MME antibody
| Reference Product | PubMed Publications |
|---|---|
| TAC1 antibody | >2 publications with MME and TAC1 |
| ICAM1 antibody | >1 publications with MME and ICAM1 |
| EDN1 antibody | >1 publications with MME and EDN1 |
| TGFB1 antibody | >1 publications with MME and TGFB1 |
| AKT1 antibody | >1 publications with MME and AKT1 |
Pathways associated with anti-MME antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| Alzheimer's Disease Pathway antibodies | Alzheimer's Disease Pathway Diagram |
| Alzheimer's Disease Pathway antibodies | Alzheimer's Disease Pathway Diagram |
| Alzheimers Disease Pathway antibodies | Alzheimers Disease Pathway Diagram |
| Hematopoietic Cell Lineage Pathway antibodies | Hematopoietic Cell Lineage Pathway Diagram |
| Hematopoietic Cell Lineage Pathway antibodies | Hematopoietic Cell Lineage Pathway Diagram |
| Metabolism Of Angiotensinogen To Angiotensins Pathway antibodies | Metabolism Of Angiotensinogen To Angiotensins Pathway Diagram |
| Metabolism Of Proteins Pathway antibodies | Metabolism Of Proteins Pathway Diagram |
| Peptide Hormone Metabolism Pathway antibodies | Peptide Hormone Metabolism Pathway Diagram |
| Protein Digestion And Absorption Pathway antibodies | Protein Digestion And Absorption Pathway Diagram |
| Protein Digestion And Absorption Pathway antibodies | Protein Digestion And Absorption Pathway Diagram |
Diseases associated with anti-MME antibody
| Disease Name | Pubmed Publications |
|---|---|
| Nervous System Diseases Antibodies | >22 publications with MME and Nervous System Diseases |
| Carcinoma Antibodies | >17 publications with MME and Carcinoma |
| Heart Diseases Antibodies | >16 publications with MME and Heart Diseases |
| Kidney Diseases Antibodies | >14 publications with MME and Kidney Diseases |
| Necrosis Antibodies | >13 publications with MME and Necrosis |
| Inflammation Antibodies | >11 publications with MME and Inflammation |
| Neoplasms, Experimental Antibodies | >11 publications with MME and Neoplasms, Experimental |
| Lung Diseases Antibodies | >10 publications with MME and Lung Diseases |
| Disease Models, Animal Antibodies | >10 publications with MME and Disease Models, Animal |
| Edema Antibodies | >7 publications with MME and Edema |
Organs/Tissues associated with anti-MME antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >55 publications with MME and Blood |
| Brain Antibodies | >23 publications with MME and Brain |
| Heart Antibodies | >21 publications with MME and Heart |
| Liver Antibodies | >21 publications with MME and Liver |
| Lung Antibodies | >18 publications with MME and Lung |
| Muscle Antibodies | >16 publications with MME and Muscle |
| Kidney Antibodies | >16 publications with MME and Kidney |
| Bone Antibodies | >12 publications with MME and Bone |
| Skin Antibodies | >9 publications with MME and Skin |
| Eye Antibodies | >9 publications with MME and Eye |