Anti-CD28 [1C6]
Référence MBS488426-5x0,2mg
Conditionnement : 5x0.2mg
Marque : MyBiosource
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Flow-cytometry using the anti-CD28 antibody 1C6 Dog peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the mouse IgG1 version of 1C6 (MBS488426, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
CD28 Recombinant Antibody – Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS488426Recombinant monoclonal antibody to CD28. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the hybridoma 1C6. More details
Product Overview
Online Datasheet in PDFProduct Name
CD28, Recombinant Antibody
Full Product Name
Anti-CD28 [1C6]
Product Gene Name
CD28 recombinant antibody
Product Synonym Names
TP44; Costimulatory molecule B7 receptor CD28; T-cell costimulatory molecule CD28
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
1C6
Species Reactivity
Dog
Form/Format
PBS with 0.02% Proclin 300.
Purity/Purification
Protein A Affinity Purified
Concentration
1mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Animal-free Manufacturing Platform
The recombinant antibody is manufactured using a recombinant, mammalian HEK293-based expression platform. The manufacturing platform is animal-free and animal component-free.
Antibody Type
Recombinant Antibody
Antibody Species
Mouse
Original Species Name
Mouse
Immunogen
Canine CD28.
Clone Note
The antibody binds specifically to canine CD28, a self self surface receptor expressed by thymocytes and a subset of natural killer cells. CD28 acts as a receptor for CD80 and CD86, providing a costimulatory signal for the activation of T-cells and the production of interleukins, such as IL-6. The 1C6 monoclonal antibody has antagonistic activity, blocking mixed lymphocyte reactions and cytotoxic T lymphocyte mediated killing. The use of the monoclonal antibody 1C6 for the neutralisation of CD26 signal following T-cell receptor activation is of clinical importance for transplantation therapy, as it has been shown to prevent graft rejection and graft-versus-host disease in the canine model of allogeneic hematopoietic cell transplantation (HCT).
Buffer Composition
PBS only with 0.02% Proclin 300.
Product Categories/Family for CD28 recombinant antibody
Transplantation; Graft Rejection; Immunology
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for CD28 recombinant antibody
Flow Cytometry (FC/FACS), FA, In Vivo, Blocking (BL), ELISA (EIA)
Flow Cytometry (FC/FACS) of CD28 recombinant antibody
Flow-cytometry using the anti-CD28 antibody 1C6 Dog peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the mouse IgG1 version of 1C6 (MBS488426, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Product References and Citations for CD28 recombinant antibody
Scott S. Graves, Diane M. Stone, Carol Loretz, Laura J. Peterson, Marina Lesnikova, Billanna Hwang, George E. Georges, Richard Nash and Rainer Storb. Antagonistic and agonistic anti-canine CD28 monoclonal antibodies: tools for allogeneic transplantation. Transplantation. 2011 Apr 27; 91(8): 833–840. PMID:21343872
Original Published Journal: Transplantation. 2011 Apr 27; 91(8): 833–840.
Original Published Journal: Transplantation. 2011 Apr 27; 91(8): 833–840.
Publication Note: Describes the role of CD28 in the activation of T cells via costimulatory signals, and the blockade of this signal using anti-CD28 monoclonal antibodies such as 1C6. Also demonstrates the advantages of using this method in comparison to cytotoxic T lymphocyte antigen 4 (CTLA-4)-Ig fusion protein by in vivo studies in the dog HCT model.
Original Published Journal: Transplantation. 2011 Apr 27; 91(8): 833–840.
Original Published Journal: Transplantation. 2011 Apr 27; 91(8): 833–840.
Publication Note: Describes the role of CD28 in the activation of T cells via costimulatory signals, and the blockade of this signal using anti-CD28 monoclonal antibodies such as 1C6. Also demonstrates the advantages of using this method in comparison to cytotoxic T lymphocyte antigen 4 (CTLA-4)-Ig fusion protein by in vivo studies in the dog HCT model.
Other Notes
Small volumes of CD28 recombinant antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CD28 purchase
MBS488426 is a recombinant antibody targeting CD28. Also known as Anti-CD28 [1C6]. Reactive in Dog.
Additional Details
NCBI/Uniprot data below describe general gene information for CD28. It may not necessarily be applicable to this product.
NCBI Official Full Name
T-cell costimulatory molecule CD28
Protein Family
T-cell-specific surface glycoprotein
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.