Anti-CD73 Antibody (3D377)
Référence NB-64-122876-100uL
Conditionnement : 100uL
Marque : Neo Biotech
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Anti-CD73 Antibody (3D377)
(Synonyms: NTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73)) Copy Product InfoSynonyms: NTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73)
Catalog No. TMAY-01316 Copy Product Info
Anti-CD73 Antibody (3D377) is a Mouse antibody targeting CD73. Anti-CD73 Antibody (3D377) can be used in ELISA,WB.
For research use only—not for human use. No sales to individuals. Use as intended only.
Product Information
Bioactivity
Related Conjugates and Formulations
Antigen Details
Stability & Storage
| Description | Anti-CD73 Antibody (3D377) is a Mouse antibody targeting CD73. Anti-CD73 Antibody (3D377) can be used in ELISA,WB. |
| Synonyms | NTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73) |
| Ig Type | Mouse IgG2b |
| Clone | 3D377 |
| Reactivity | Human |
| Specificity | Human 5'-nucleotidase/CD73/NT5E No cross-reactivity in ELISA with Human cell lysate (293 cell line) |
| Verified Activity | Anti-NT5E mouse monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:63 kDa. -Observed band size:63 kDa |
| Application | ELISA WB |
| Recommended Dose | WB: 1:500-1:1000; ELISA: 1:5000-1:10000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD73 / NT5E (rh CD73 / NT5E; TMPY-01851; NP_002517.1; Met 1-Lys 547). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | 5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5'-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signaling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purinergic receptors to induce cellular responses. CD73 has also been reported to regulate the expression of pro-inflammatory molecules in mouse endothelium. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human CD73 / NT5E Protein (TMPY-01851) |
| Antigen Species | Human |
| Biology Area | Phosphatases and Regulators |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Keywords
NT-5NT 5CD-73CD 735'-nucleotidase, ecto
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