Anti-CFTR Antibody

Référence CPA3849-100ul

Conditionnement : 100ul

Marque : Cohesion Biosciences

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  • Western blot analysis of CFTR expression in HEK293T (A), Hela (B), rat spleen (C), rat kidney (D) whole cell lysates. (Predicted band size: 168 kD; Observed band size: 168; 90 kD)
  • Immunohistochemical analysis of CFTR staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Western blot analysis of CFTR expression in wild type (WT) and knockdown (KD) THP1 cell lysates.
Product Name:Anti-CFTR Antibody [KO/KD Validated]
Cat No:CPA3849
Source:Rabbit
Reactivity:H, M, R, Mk, Rb
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description:Rabbit polyclonal antibody to CFTR
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CFTR. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Specificity:Recognizes endogenous levels of CFTR protein.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)
Gene Symbol:CFTR
Alternative Names:ABCC7; Cystic fibrosis transmembrane conductance regulator; CFTR; ATP-binding cassette sub-family C member 7; Channel conductance-controlling ATPase; cAMP-dependent chloride channel
Entrez Gene (Human): 1080;
Entrez Gene (Mouse): 12638;
Entrez Gene (Rat): 24255;
SwissProt (Human): P13569;
SwissProt (Mouse): P26361;
SwissProt (Rat): P34158;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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