Anti-Chordin Polyclonal Antibody

Référence NB-64-154274-50uL

Conditionnement : 50uL

Marque : Neo Biotech

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Anti-Chordin Polyclonal Antibody

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Synonyms:

Catalog No. TMAB-04312 Copy Product Info
Anti-Chordin Polyclonal Antibody is a Rabbit antibody targeting Chordin. Anti-Chordin Polyclonal Antibody can be used in WB.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Description
Anti-Chordin Polyclonal Antibody is a Rabbit antibody targeting Chordin. Anti-Chordin Polyclonal Antibody can be used in WB.
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep)
Verified Activity
1. Sample:
HepG2 (Human) Cell Lysate at 30 μg
U2OS (Human) Cell Lysate at 30 μg
MDA-MB-231 (Human) Cell Lysate at 30 μg
293T (Human) Cell Lysate at 30 μg
Primary: Anti-Chordin (TMAB-04312) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99 kD
Observed band size: 99 kD
2. Sample:
HepG2 (Human) Cell Lysate at 30 μg
U2OS (Human) Cell Lysate at 30 μg
MCF-7 (Human) Cell Lysate at 30 μg
293T (Human) Cell Lysate at 30 μg
Primary: Anti-Chordin (TMAB-04312) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99 kD
Observed band size: 96 kD
Application
WB
Recommended Dose
WB: 1:500-2000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted.
Tissue SpecificityExpressed at the highest level in liver.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe patterning of the CNS relies on the interaction of multiple signaling molecules such as Sonic Hedgehog, Wnts, and BMPs and their antagonists Chordin and Noggin. At midgastrula, expression of Noggin overlaps that of Chordin. Chordin is a key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta-like bone morphogenetic proteins and sequestering them in latent complexes. The gene which encodeschordin maps to human chromosome 3q27. Noggin is a secreted protein that binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily of signaling proteins, such as BMP-2,4,7. The gene which encodes noggin maps to human chromosome 17q22.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Chordin
Antigen Species
Human
Gene Name
CHRD
Gene ID
8646
Protein Name
Chordin
Uniprot ID
Q9H2X0
Function
Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes.
Chemical Properties
Molecular WeightTheoretical: 99 kDa. Actual: 99 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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