fr en

Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.

Anti-Fibrillarin Antibody

Imprimer

Référence 560-FIBt

Conditionnement : 25uL

Contactez votre distributeur local :

Téléphone : +1 850 650 7790

Marque : Phosphosolutions

Demander plus d'informations

Contactez votre distributeur local :

Téléphone : +1 850 650 7790

Anti-Fibrillarin Antibody (38F3)

Our Anti-Fibrillarin primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Fibrillarin and is Protein G purified. It is great for use in WB, IHC, ICC.

High magnification confocal image of HeLa cells showing strong, prominent labeling of the nucleoli in the nucleus with Anti-Fibrillarin (Cat. 560-FIB, 1:100, green), colabeled with Anti-Vimentin (Cat. 2105-VIM, red, 1:1000) showing the cytoplasmic intermediated filaments, and nuclear staining was done with DAPI (blue).

Western blot of HeLa cell lysate showing specific immunolabeling of the ~34 kDa fibrillarin protein.

Immunofluorescence of human neuroblastoma SH-SY5Y cells showing strong, prominent labeling of the nucleoli in the nucleus with Anti-Fibrillarin (Cat. 560-FIB, 1:500, green), and nuclear staining was done with DAPI (blue) which makes the fibrillarin marker appear pale blue.

High magnification confocal image of HeLa cells showing strong, prominent labeling of the nucleoli in the nucleus with Anti-Fibrillarin (Cat. 560-FIB, 1:100, green), colabeled with Anti-Vimentin (Cat. 2105-VIM, red, 1:1000) showing the cytoplasmic intermediated filaments, and nuclear staining was done with DAPI (blue).

Click on image to zoom

Species Reactivity

Bovine, Chicken, Drosophila, Human, Mouse, Pig, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Go to Full Product Details

Data Sheet

Trial Size

SKU: 560-FIB

Ships: 1-2 business days

Product Details

Target

Fibrillarin

Scientific Background

Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli (1). The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archaea generally called fibrillarin (2). Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34 kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable (3). This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).

Format

Protein G Purified

Clonality

Monoclonal

Clone

38F3

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

NOP1

Molecular Weight

34 kDa

Immunogen

Yeast nuclear preparation

Immunogen Species

Yeast

Species Reactivity

Bovine, Chicken, Drosophila, Human, Mouse, Pig, Rat

Expected Reactivity

Canine, Feline, Goat, Guinea Pig, Hamster, Horse, Non-Human Primate, Rabbit, Sheep, Vole

Antibody Registry ID

AB_2492093

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Protein G purified culture supernatant.

Buffer

PBS + 50% glycerol and 5 mm NaN3

Dilution Ranges

WB: 1:1000
IHC: 1:100-1:500
ICC: 1:100-1:500

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~34 kDa Fibrillarin /Nop1p protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

34 kD nucleolar scleroderma antigen antibody, 34 kDa nucleolar scleroderma antigen antibody, Fbl antibody, FBRL_HUMAN antibody, FIB antibody, FIB1 antibody, FLRN antibody, Histone-glutamine methyltransferase antibody, Nop1p antibody, RNA U3 small nucleolar interacting protein 1 antibody, RNU3IP1 antibody, rRNA 2' O methyltransferase fibrillarin antibody, rRNA 2'-O-methyltransferase fibrillarin antibody

UniProt ID

P15646

NCBI Gene ID

851548

Shipping

Blue Ice

Western Blot Protocol: Download

General References

Paeschke, K., Simonsson, T., Postberg, J., Rhodes, D. and Lipps, H.J., 2005. Telomere end-binding proteins control the formation of G-quadruplex DNA structures in vivo. Nature Structural & Molecular Biology, 12(10), p.847. PMID: 16142245
Vermaak, D., Henikoff, S. and Malik, H.S., 2005. Positive selection drives the evolution of rhino, a member of the heterochromatin protein 1 family in Drosophila. PLoS Genetics, 1(1), 96-108. PMID: 16103923
Tyagi, S. and Alsmadi, O., 2004. Imaging native β-actin mRNA in motile fibroblasts. Biophysical Journal, 87(6), pp.4153-4162. PMID: 15377515
Newton, K., Petfalski, E., Tollervey, D. and Cáceres, J.F., 2003. Fibrillarin is essential for early development and required for accumulation of an intron-encoded small nucleolar RNA in the mouse. Molecular and Cellular Biology, 23(23), pp.8519-8527. PMID: 14612397
Aris, J.P. and Blobel, G., 1988. Identification and characterization of a yeast nucleolar protein that is similar to a rat liver nucleolar protein. The Journal of Cell Biology, 107(1), pp.17-31. PMID: 3292539
Ochs, R.L., Lischwe, M.A., Spohn, W.H. and Busch, H., 1985. Fibrillarin: a new protein of the nucleolus identified by autoimmune sera. Biology of the Cell, 54(2), pp.123-133. PMID: 2933102

Anti-Fibrillarin Antibody

Référence 560-FIBt

Contactez votre distributeur local :

Marque : Phosphosolutions

Contactez votre distributeur local :

Anti-Fibrillarin Antibody (38F3)

SKU: 560-FIB

Product Details

General References

Bulk Order Anti-Fibrillarin Antibody