Anti-Histone H3 Polyclonal Antibody
Référence OAAI00717
Conditionnement : 100ug
Marque : Aviva Systems Biology
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Anti-Histone H3 Polyclonal Antibody (OAAI00717)
| Datasheets/Manuals | Printable datasheet for Anti-Histone H3 Polyclonal Antibody (OAAI00717) |
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| Predicted Species Reactivity | Bovine|Chicken|Human|Mouse|Rat |
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| Product Format | Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Application | ELISA, FC, IHC-Fr, IHC-P, WB |
| Additional Information | Background: Histones are basic nuclear proteins that together with DNA make up the nucleosome structure in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4. The sequence is found in all mammals and a wide range of species, including D. melanogaster, Arabidopsis, Chicken and Xenopus. |
| :: | Positive Control: Hela Nuclear Extract |
| :: | Cellular Location: Nucleus |
| Reconstitution and Storage | The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles. |
| Immunogen | A synthetic peptide within ~100 amino acids from N-terminus of human histone H3. |
| Purification | Affinity Purified |
| Concentration | 100ug/200ul |
| Specificity | H3C1 |
| Formulation | This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer |
| Application Info | WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested |
| Reference | Vakoc C et al. Histone H3 Lysine 9 Methylation and HP1γ Are Associated with Transcription Elongation throμgh Mammalian Chromatin . Molecular Cell (2003), Volume 19 , Issue 3 , Pages 381 - 391. David J Seward, et al. Demethylation of trimethylated histone H3 Lys4 in vivo by JARID1 JmjC proteins. Nature Structural & Molecular Biology 14, 240 - 242 (2007) |
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| Gene Symbol | H3C1 |
| Gene Full Name | H3 clustered histone 1 |
| Alias Symbols | H3 histone family, member A;H3/A;H3C10;H3C11;H3C12;H3C2;H3C3;H3C4;H3C6;H3C7;H3C8;H3FA;HIST1H3A;histone 1, H3a;histone cluster 1 H3 family member a;histone cluster 1, H3a;histone H3.1;histone H3/a. |
| NCBI Gene Id | 8350 |
| Description of Target | Histones are basic nuclear proteins that together with DNA make up the nucleosome structure in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4. The sequence is found in all mammals and a wide range of species, including D. melanogaster, Arabidopsis, Chicken, and Xenopus |
| Uniprot ID | P68431 |
| Molecular Weight | 17 |
