Purified Recombinant Human Interleukin1 Beta (>98%)

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Lyophilized

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at 20°C to 70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 28°C. For longterm storage of the reconstituted antibody, aseptically aliquot into working volumes and store at 20°C to 70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

Immunohistochemistry: Suitable for use at concentration of 115 µg/mL.
Immunocytochemistry: Suitable at concentration of 515 µg/mL.
Neutralization: Measured by its ability to neutralize IL‑1 beta /IL‑1F2induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND₅₀) is typically 642 ng/mL in the presence of 50 pg/mL Recombinant Human IL‑1 beta /IL‑1F2.

Rabbit AntiHuman Interleukin1 Beta recognizes Human Interleukin1 Beta. This antigen affinity purified polyclonal antibody was purified using immobilized antigen agarose chromotography.

IL1R1 is an 80 kD transmembrane glycoprotein and a member of the Ig superfamily. IL1 binds both IL1R1 and IL1R2. The ligands IL1α and IL1β only commence when binding IL1R1 because IL1R2 acts as a decoy receptor and competitively antagonizes the binding of IL1α and IL1β, thereby reducing their biological effects. Binding of the ligands to IL1R1 is enhanced by an accessory protein, IL1RAcP. IL1α can exist as either a 31 kD precursor or an 18 kD mature form. IL1α is a proinflammatory cellassociated cytokine and IL1β is a 17.5 kD proinflammatory secreted cytokine. Both IL1α and IL1β bind to the same receptor and have similar properties that include stimulation of thymocyte proliferation via IL2 release, Bcell maturation and proliferation, mitogenic FGFlike activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. Furthermore, IL1β mediates a variety of immune and inflammatory responses. IL1β is a major cause of severe inflammation in the mouse model of Crohn’s Disease, which makes IL1β an important target for this disease.

1. Dinarello, CA. et al. (1996) Blood 87: 2095
2. Yuan, J. et al. (1993) Cell 75: 641
3. Maeda, S. et al. (2005) Science 4: 735