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> Anti-LILRB1 Antibody (9N307)

Anti-LILRB1 Antibody (9N307)

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Référence NB-64-158244-50uL

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Anti-LILRB1 Antibody (9N307)

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Catalog No. TMAB-08305 Copy Product Info
Anti-LILRB1 Antibody (9N307) is a Rabbit antibody targeting LILRB1. Anti-LILRB1 Antibody (9N307) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Description
Anti-LILRB1 Antibody (9N307) is a Rabbit antibody targeting LILRB1. Anti-LILRB1 Antibody (9N307) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Ig Type
IgG
Clone
9N307
Reactivity
Human
Verified Activity
1. The THP-1 (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-LILRB1 antibody (TMAB-08305, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
2. 4% Paraformaldehyde-fixed THP-1 (H) cell; Antibody incubation with (LILRB1) monoclonal Antibody, unconjugated (TMAB-08305) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
3. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LILRB1 Monoclonal Antibody, Unconjugated (TMAB-08305) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. 25 ug total protein per lane of various lysates (see on figure) probed with LILRB1 monoclonal antibody, unconjugated (TMAB-08305) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
Application
WB
IHC-P
IHC-Fr
ICC/IF
IF
FCM
Recommended Dose
WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane; Single-pass type I membrane protein.
Tissue SpecificityExpressed on monocytes and B-cells, and at lower levels on dendritic cells. Detected at low levels in natural killer (NK) cells.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human LILRB1
Antigen Species
Human
Gene Name
LILRB1
Gene ID
10859
Protein Name
Leukocyte immunoglobulin-like receptor subfamily B member 1
Uniprot ID
Q8NHL6
Chemical Properties
Molecular WeightTheoretical: 72 kDa. Actual: 110 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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