Anti-MEK1 Antibody
Référence OAED00083
Conditionnement : 100ug
Marque : Aviva Systems Biology
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Anti-MEK1 Antibody (OAED00083)
| Datasheets/Manuals | Printable datasheet for Anti-MEK1 Antibody (OAED00083) |
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| Predicted Species Reactivity | Human|Mouse|Rat|Xenopus |
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| Clonality | Polyclonal |
| Host | Rabbit |
| Application | Immunofluorescence|Immunohistochemistry|Western blot |
| Reconstitution and Storage | Long Term Storage : -20C |
| Immunogen | Synthetic peptide corresponding to the sequence near the N-terminus of human MEK1. The sequence is completely conserved in mouse, rat, hamster, rabbit and Xenopus. |
| Specificity | Recognizes human, mouse, rat and Xenopus MEK1. Detect a band of ~48kDa by Western blot. |
| Formulation | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
| Purity | Protein A affinity purified. |
| Application Info | Immunofluorescence Immunohistochemistry (1:50) Western Blot (1μg/ml, colorimetric) Optimal conditions must be determined individually for each application. |
| Reference | Role of ether-linked lysophosphatidic acids in ovarian cancer cells: Y. Xu, et al. ; J. Lipid Res. 43, 463 (2002), Application(s): WB using human samples, Abstract; |
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| Gene Symbol | MAP2K1 |
| Gene Full Name | mitogen-activated protein kinase kinase 1 |
| Alias Symbols | CFC3;dual specificity mitogen-activated protein kinase kinase 1;ERK activator kinase 1;MAPK/ERK kinase 1;MAPKK 1;MAPKK1;MEK 1;MEK1;MEL;MKK1;PRKMK1;protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1). |
| NCBI Gene Id | 5604 |
| Protein Name | Dual specificity mitogen-activated protein kinase kinase 1 |
| Description of Target | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. |
| Uniprot ID | Q02750 |
