General Information
| Short Description | Rabbit polyclonal anti-Phospho-Calcium/calmodulin-dependent protein kinase type II subunit alpha and Calcium/calmodulin-dependent protein kinase type II subunit delta-Thr286 (256-305 aa) for use in WB, IHC, IF and ELISA in Human, Mouse, Rat and Pig s |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat/Pig |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:10000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | |
| Gene ID | |
| Uniprot ID | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human CaMK2 around the phosphorylation site of Thr286 at the amino acid range 256-305 |
| Immunogen Region | 256-305 aa |
| Specificity | Phospho-CaMKII Alpha/Delta (T286) Polyclonal Antibody detects endogenous levels of CaMKII Alpha/Delta protein only when phosphorylated at T286. |
Additional Info
| Function | Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission. Regulates dendritic spine development. Also regulates the migration of developing neurons. Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity. Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity. |
| Protein Name | Calcium/Calmodulin-Dependent Protein Kinase Type Ii Subunit AlphaCam Kinase Ii Subunit AlphaCamk-Ii Subunit Alpha |
| Database Links | |
| Cellular Localisation | Cell JunctionSynapsePostsynaptic DensityCell ProjectionDendritic SpineDendritePostsynaptic Lipid Rafts |
| Alternative Antibody Names | Anti-Calcium/Calmodulin-Dependent Protein Kinase Type Ii Subunit Alpha antibodyAnti-Cam Kinase Ii Subunit Alpha antibodyAnti-Camk-Ii Subunit Alpha antibodyAnti-CAMK2A antibodyAnti-CAMKA antibodyAnti-KIAA0968 antibody |
Information sourced from Uniprot.org