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> Anti-Phospho-ERK1/2 (Thr185) Antibody (1B497)

Anti-Phospho-ERK1/2 (Thr185) Antibody (1B497)

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Référence NB-64-118559-50uL

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Anti-Phospho-ERK1/2 (Thr185) Antibody (1B497)

(Synonyms: PRKM3, Phospho-ERK1/2 (T185), p-ERK1/2 (Thr185), p-ERK1/2 (T185), p44-MAPK, P44MAPK, p44-ERK1, P44ERK1, HUMKER1A, HS44KDAP, ERT2, ERK-2, Erk2, ERK1/2 (p-Thr185), ERK1/2 (p-T185), ERK-1, ERK1) Copy Product Info

Synonyms: PRKM3, Phospho-ERK1/2 (T185), p-ERK1/2 (Thr185), p-ERK1/2 (T185), p44-MAPK, P44MAPK, p44-ERK1, P44ERK1, HUMKER1A, HS44KDAP, ERT2, ERK-2, Erk2, ERK1/2 (p-Thr185), ERK1/2 (p-T185), ERK-1, ERK1

Catalog No. TMAC-03213 Copy Product Info
Anti-Phospho-ERK1/2 (Thr185) Antibody (1B497) is a Rabbit antibody targeting Phospho-ERK1/2 (Thr185). Anti-Phospho-ERK1/2 (Thr185) Antibody (1B497) can be used in FCM,ICC,IHC,WB.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity
Description
Anti-Phospho-ERK1/2 (Thr185) Antibody (1B497) is a Rabbit antibody targeting Phospho-ERK1/2 (Thr185). Anti-Phospho-ERK1/2 (Thr185) Antibody (1B497) can be used in FCM,ICC,IHC,WB.
SynonymsPRKM3, Phospho-ERK1/2 (T185), p-ERK1/2 (Thr185), p-ERK1/2 (T185), p44-MAPK, P44MAPK, p44-ERK1, P44ERK1, HUMKER1A, HS44KDAP, ERT2, ERK-2, Erk2, ERK1/2 (p-Thr185), ERK1/2 (p-T185), ERK-1, ERK1
Ig Type
IgG
Clone
1B497
Reactivity
Human
Verified Activity
1. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Phospho-Erk1 (T202)+Erk2 (T185) antibody. Counter stained with hematoxylin.
2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-Erk1 (T202)+Erk2 (T185) antibody. Counter stained with hematoxylin.
3. ICC staining Phospho-Erk1 (T202)+Erk2 (T185) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining Phospho-Erk1 (T202)+Erk2 (T185) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining Phospho-Erk1 (T202)+Erk2 (T185) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. Flow cytometric analysis of MCF-7 cells with Phospho-Erk1 (T202)+Erk2 (T185) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody.
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Application
FCM
ICC
IHC
WB
Recommended Dose
WB: 1:1000; IHC: 1:50-200; ICC: 1:100-500; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundMitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines, and research investigators consider it an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors, such as U0126 and PD98059.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
Phospho
Antigen Details
Immunogen
A synthesized phosphopeptide: human Erk2 around the phosphorylation site of Thr185
Antigen Species
Human
Uniprot ID
P27361 & P28482
Chemical Properties
Molecular WeightTheoretical: 42/44 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Keywords

pERK1/2 (Thr185)pERK1/2 (T185)ERK1/2 (pThr185)ERK1/2 (pT185)ERK1/2 (phospho-Thr185)ERK1/2 (phospho-T185)
Related Tags: Anti-Phospho-ERK1/2 (Thr185) Antibody (1B497) molecular weight