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Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.

Anti-Sox2 Antibody

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Référence SM5511

Conditionnement : 100uL

Contactez votre distributeur local :

Téléphone : +1 850 650 7790

Marque : Phosphosolutions

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Contactez votre distributeur local :

Téléphone : +1 850 650 7790

Anti-Sox2 Antibody (M551)

Our Sox2 primary antibody from PhosphoSolutions is mouse monoclonal. It detects chicken, human, mouse, rat, and Xenopus Sox2 and is Protein G purified. It is great for use in WB, ICC.

Western blot image of mouse F9 stem cells treated with with calyculin A (100 nM, 30 min.) (lanes 1-4) then Sox2 was dephosphorylated with lambda phosphatase (lanes 2 & 4). The blot was probed with mouse monoclonal Sox2 (lanes 1 & 2) and rabbit polyclonal anti-Sox2 (Thr-118) phospho-specific antibody (lanes 3 & 4).

Immunocytochemical labeling of Sox2 in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Sox2 (SM5511). The antibody was detected using goat anti-mouse DyLight® 594.

Western blot image of mouse F9 stem cells treated with with calyculin A (100 nM, 30 min.) (lanes 1-4) then Sox2 was dephosphorylated with lambda phosphatase (lanes 2 & 4). The blot was probed with mouse monoclonal Sox2 (lanes 1 & 2) and rabbit polyclonal anti-Sox2 (Thr-118) phospho-specific antibody (lanes 3 & 4).

Click on image to zoom

Species Reactivity

Chicken, Frog, Human, Mouse, Rat

Applications

ICC, WB

Host Species

Mouse

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SKU: SM5511

Ships: 1-2 business days

Product Details

Target

Sox2

Scientific Background

Embryonic stem cells can maintain a pluripotent state that is controlled by a set of transcription factors that include Oct-4, Sox2, and Nanog. Chromatin immunoprecipitation experiments show that Sox2 and Oct-4 bind to thousands of gene regulatory sites, many of which regulate cell pluripotency and early embryonic development. siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency, while overexpression of Oct-4 and Sox2, along with additional transcription factors Klf4 and c-Myc, can reprogram somatic cells to a pluripotent state. Sox2 also regulates adult multipotent progenitors in various epithelial tissues, and may be important for survival and regeneration of these tissues. The activity of Sox2 may be regulated by phosphorylation and methylation. Akt1 phosphorylates Thr-118 and enhances Sox2 transcriptional activity, while Set7 can monomethylate Lys-119 leading to inhibition of Sox2 transcriptional activity, as well as Sox2 ubiquination and degradation. In addition, Sox2 Thr-128 is constituitively phoshorylated in the F9 mouse stem cell line.

Format

Protein G Purified

Clonality

Monoclonal

Clone

M551

Isotype

IgG2b

Applications

ICC, WB

Host Species

Mouse

Gene Name

SOX2

Molecular Weight

Immunogen

Clone M551 was generated from a recombinant protein corresponding to the full length sequence from human Sox2. This sequence is highly conserved in rat and mouse Sox2.

Immunogen Species

Human

Species Reactivity

Chicken, Frog, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500
ICC: 1:50

Conjugate

Unconjugated

Specificity

This antibody antibody detects a 34 kDa* protein on SDS-PAGE immunoblots of mouse F9 stem cells, and detects a full length recombinant human Sox2 protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

SRY (sex determining region Y) box-2, ANOP3, MCOPS3, SOX-2

UniProt ID

P48431

Shipping

Blue Ice

Anti-Sox2 Antibody

Référence SM5511

Contactez votre distributeur local :

Marque : Phosphosolutions

Contactez votre distributeur local :

Anti-Sox2 Antibody (M551)

SKU: SM5511

Product Details

Bulk Order Anti-Sox2 Antibody