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Anti-SPO11 Polyclonal Antibody

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Référence NB-64-163050-100uL

Conditionnement : 100uL

Marque : Neo Biotech

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Anti-SPO11 Polyclonal Antibody

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Synonyms:

Catalog No. TMAB-13128 Copy Product Info
Anti-SPO11 Polyclonal Antibody is a Rabbit antibody targeting SPO11. Anti-SPO11 Polyclonal Antibody can be used in WB.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity
Description
Anti-SPO11 Polyclonal Antibody is a Rabbit antibody targeting SPO11. Anti-SPO11 Polyclonal Antibody can be used in WB.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig,Horse)
Verified Activity
Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Rat Testis tissue lysates
Lane 3: Human PC-3 cell lysates
Primary: Anti-SPO11 (TMAB-13128) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 48 kDa
Application
WB
Recommended Dose
WB: 1:500-2000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNuclear
Tissue SpecificityHighly expressed in testis.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundSpo11 is a type II topoisomerase that is thought to generate the chromosome breaks that initiate meiotic recombination. The Spo11 protein initiates meiotic recombination by generating DNA double-strand breaks (DSBs) and is required for meiotic synapsis in S. cerevisiae. The DSBs are located mostly in promoter regions, where the chromatin is in an open configuration, and cluster in domains along the chromosome. Expression of the Spo11 is detected mainly in the testis, in agreement with its predicted function in the initiation of meiotic recombination. Disruption of Spo11 leads to severe gonadal abnormalities from defective meiosis and results in infertility.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human SPO11
Antigen Species
Human
Gene Name
SPO11
Gene ID
23626
Protein Name
Meiotic recombination protein SPO11
Uniprot ID
Q9Y5K1
Function
Required for meiotic recombination. Mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination (By similarity). Essential for the phosphorylation of SMC3, HORMAD1 and HORMAD2 (By similarity).
Chemical Properties
Molecular WeightTheoretical: 45 kDa. Actual: 48 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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