Orb623174-1l | Antigen Coating Buffer, 5X Biovalley

Antigen Coating Buffer, 5X

SKU: orb623174

Description

Antigen Coating Buffer maximizes the adsorption of antigens onto polystyrene plates and stabilizes their 3-D structure to enhance specific signal, reduce reagent consumption, and extend the shelf-life of coated plates.

Research Area

Immunology & Inflammation

Images & Validation

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Application Notes	1. Coat antibody or antigen onto the ELISA plate using ICT’s Antibody Coating Buffer or Antigen Coating Buffer. 2. Incubate 8–24 hours at room temperature (RT). 3. Aspirate the coating solution. 4. Wash each well twice with ICT’s ELISA Wash Buffer. 5. Block the uncoated regions of the ELISA plate by pipetting 300–400 µL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution. 6. Incubate 8–24 hours at RT. For best blocking, incubate overnight at RT. 7. Aspirate the blocking buffer; do not wash. 8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8°C.

Application Notes

1. Coat antibody or antigen onto the ELISA plate using ICT’s Antibody Coating Buffer or Antigen Coating Buffer. 2. Incubate 8–24 hours at room temperature (RT). 3. Aspirate the coating solution. 4. Wash each well twice with ICT’s ELISA Wash Buffer. 5. Block the uncoated regions of the ELISA plate by pipetting 300–400 µL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution. 6. Incubate 8–24 hours at RT. For best blocking, incubate overnight at RT. 7. Aspirate the blocking buffer; do not wash. 8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8°C.

Key Properties

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Storage & Handling

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Storage	Room temp
Concentration	5 X
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Antigen Coating Buffer, 5X [orb1821207]
Sizes Available: 500 ml
Antigen Coating Buffer, 5X [orb1821208]
Sizes Available: 100 ml

Antigen Coating Buffer, 5X

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Marque : Biorbyt

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Antigen Coating Buffer, 5X

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Antigen Coating Buffer, 5X [orb1821207]

Antigen Coating Buffer, 5X [orb1821208]

Protocol Information