Orb1806523-48 | ASD(Androstenedione) ELISA Kit Biovalley

ASD(Androstenedione) ELISA Kit

Catalog Number	orb1806523
Category	Assays and Kits
Description	ASD(Androstenedione) ELISA Kit
Reactivity	All
Range	156.25-10000 pg/mL
Target	ASD
Sensitivity	48.8 pg/mL
Alternative names	4-Androstenedione; 4-Androstene-3,17-Dione
Note	For research use only
Application notes	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Androstenedione(ASD) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Androstenedione(ASD). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Androstenedione(ASD) in the samples is then determined by comparing the OD of the samples to the standard curve.
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Expiration Date	Please enquire.