ASPH ELISA Kit (Human)
Référence OKEH04669
Conditionnement : 96Wells
Marque : Aviva Systems Biology
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ASPH ELISA Kit (Human) (OKEH04669)
| Datasheets/Manuals | Printable datasheet for ASPH ELISA Kit (Human) (OKEH04669) |
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| COA Datasheet | Printable COA datasheet for OKEH04669 |
| Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology ASPH ELISA Kit (Human) (OKEH04669) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ASPH has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for ASPH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ASPH captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 95% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-Assay CV: < 4.0% Mean Inter-Assay CV: < 7.5% | ||||||||||||||||||||||
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 0.053 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Human Aspartyl/asparaginyl beta-hydroxylase | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | ASPH |
|---|---|
| Gene Full Name | aspartate beta-hydroxylase |
| Alias Symbols | A beta H-J-J, AAH, ASP beta-hydroxylase, Aspartate beta-hydroxylase, aspartyl/asparaginyl beta-hydroxylase, BAH, cardiac junctin, CASQ2BP1, FDLAB, HAAH, humbug, JCTN, junctate, junctin, peptide-aspartate beta-dioxygenase. |
| NCBI Gene Id | 444 |
| Protein Name | Aspartyl/asparaginyl beta-hydroxylase |
| Description of Target | Isoform 1: specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins. |
| Uniprot ID | Q12797 |
| Protein Accession # | NP_001158222.1 |
| Nucleotide Accession # | NM_001164750.1 |
| Protein Size (# AA) | 758 |
| Molecular Weight | 86 KDa |
