Basic Cytotoxicity Test BioAssay™ Kit
Référence 474004-250T
Conditionnement : 250Tests
Marque : US Biological
Applications
FCShipping Temp
Blue IceStorage Temp
4°C/-20°CSample Type:
Cell culture
Reagent Name:
7-AAD, CFSE
Target:
Cell-mediated cytotoxicity
Excitation/Emission:
7-AAD–546nm/647nm; CFSE–492nm/520nm
Method of Analysis:
Flow cytometry
Intended Use:
Basic Cytotoxicity Test Assay is a single-tube, dual-color assay for determining cytotoxicity by flow cytometry. The BioAssay™ employs a green fluorescent cellular stain, CFSE, to label target cells and the red live/dead viability dye, 7-AAD, to identify the dead cells present in the cytotoxicity BioAssay™ samples. Analyze your results using a flow cytometer.
Test Principle:
Cytolytic activity is an important process for eliminating intracellular pathogens and cancer cells. Basic Cytotoxicity Assay allows you to assess cytolytic activity in cell culture.
The Basic Cytotoxicity BioAssay™ includes two fluorescent reagents: CFSE and 7-AAD. CFSE, a green fluorescing cellular stain, is utilized to identify the target cell population. The unstained effector cells are added and incubated with the target cells. 7-AAD, a red fluorescing live/dead stain, is then added to stain all necrotic cells red by binding to the DNA of membrane-compromised cells. The target cell and effector cell populations can then be easily distinguished, and flow cytometry easily identifies live and necrotic cells.
Kit Components:
125 tests:
CFSE target cell stain, 1 vial
7-AAD vital stain, 1 vial
10X Assay Buffer, 1x30mL
CFSE target cell stain, 1 vial
7-AAD vital stain, 1 vial
10X Assay Buffer, 1x30mL
250 tests:
CFSE target cell stain, 1 vial
7-AAD vital stain, 2 vials
10X Assay Buffer, 1x60mL
7-AAD vital stain, 2 vials
10X Assay Buffer, 1x60mL
Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards