Blocking Buffer Optimization Pack: 100mL of BB1, BB2, BB3
Référence MBS258062-5x100mL
Conditionnement : 5x100mL
Marque : MyBiosource
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Blocking Buffer Optimization Pack: 100mL of BB1, BB2, BB3 Accessory Reagent – Supplied at 1X.
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS258062Description: Receive our three most popular Blocking Buffer formulations (General Block, Neptune Block, SynBlock) in this economical pack to evaluate the best blocking buffer for a particular assay.Background: After coating the EIA/RIA plate with the capture… More details
Product Overview
Online Datasheet in PDFProduct Name
Blocking Buffer Optimization Pack: 100mL of BB1, BB2, BB3, Accessory Reagent
Full Product Name
Blocking Buffer Optimization Pack: 100mL of BB1, BB2, BB3
Product Synonym Names
Block Buffer Optimization Pack
Form/Format
Supplied at 1X.
Preparation and Storage
Store at 2-8 degree C.
pH
7.4 at 1X
Contains
SynBlock-all synthetic materials; General Block-BSA; Neptune Block-non-mammalian proteins
Product Categories/Family for Blocking Buffer Optimization Pack: 100mL of BB1, BB2, BB3 accessory reagent
ELISA Solutions; Blocking Buffers
Application Notes for Blocking Buffer Optimization Pack: 100mL of BB1, BB2, BB3 accessory reagent
1. Coat antibody or antigen onto the ELISA plate using ICT's Antibody Coating Buffer or Antigen Coating Buffer.
2. Incubate 8-24 hours at room temperature (RT).
3. Aspirate the coating solution.
4. Wash each well twice with ICT's ELISA Wash Buffer.
5. Block the uncoated regions of the ELISA plate by pipetting 300-400 uL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution.
6. Incubate 8-24 hours at RT. For best blocking, incubate overnight at RT.
7. Aspirate the blocking buffer; do not wash.
8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8 degree C.
2. Incubate 8-24 hours at room temperature (RT).
3. Aspirate the coating solution.
4. Wash each well twice with ICT's ELISA Wash Buffer.
5. Block the uncoated regions of the ELISA plate by pipetting 300-400 uL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution.
6. Incubate 8-24 hours at RT. For best blocking, incubate overnight at RT.
7. Aspirate the blocking buffer; do not wash.
8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8 degree C.
Other Notes
Small volumes of Blocking Buffer Optimization Pack: 100mL of BB1, BB2, BB3 accessory reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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MBS258062 is an accessory reagent targeting Blocking Buffer Optimization Pack: 100mL of BB1, BB2, BB3.