Bovine Serum
Référence MBS258540-20mL
Conditionnement : 20mL
Marque : MyBiosource
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Serum Reagent
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS258540This serum is used as blocking agents at 5% in buffer with all Immunoassays, to reduce non-specific background and conserved sequence. This serum can also be used as a negative control in immunoassays with proper dilution.It is recommended that the diluted… More details
Product Overview
Product Name
Serum, Reagent
Full Product Name
Bovine Serum
Preparation and Storage
Store at 2-8 degree C
Product Categories/Family for Serum reagent
Applications Tested/Suitable for Serum reagent
Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes for Serum reagent
For Immunohistochemistry (IHC) and Immunocytochemistry (ICC)
1. If tissues are used, deparafinize and hydrate tissue sections through xylene or other clearing agents and graded alcohols.(For frozen sections or cell smears; use unfixed, acetone fixed or appropriate fixative for the antigen in question; for cell smears it may be necessary to permealize the cell by detergent, please refer to antibody protocol.
2.Wash 2-3 with distilled or deionized water.
3.Incubate sections/cell smear in Endoblocker (#1) for 5-10 minutes at room temperature or 37 degree C. Note: If antigen retriever is required it can be applied after this stage.
4.Wash slide with PBS or Tris saline (with 0.02-0.05% nonionic detergent, Triton X100, Tween 20 or NP-40) or washing buffer (Immuno Automation buffer IBSC ) 3-5X.
5.Incubate sections/cell smear in Protein blocking solution (diluted animal serum), for 10 minutes. at RT or 37 degree C.
6. Wash slide with PBS 1X.
7. Follow instructions for IHC/ICC.The diluted serums are also used as blocking agents in all immunoassays.
1. If tissues are used, deparafinize and hydrate tissue sections through xylene or other clearing agents and graded alcohols.(For frozen sections or cell smears; use unfixed, acetone fixed or appropriate fixative for the antigen in question; for cell smears it may be necessary to permealize the cell by detergent, please refer to antibody protocol.
2.Wash 2-3 with distilled or deionized water.
3.Incubate sections/cell smear in Endoblocker (#1) for 5-10 minutes at room temperature or 37 degree C. Note: If antigen retriever is required it can be applied after this stage.
4.Wash slide with PBS or Tris saline (with 0.02-0.05% nonionic detergent, Triton X100, Tween 20 or NP-40) or washing buffer (Immuno Automation buffer IBSC ) 3-5X.
5.Incubate sections/cell smear in Protein blocking solution (diluted animal serum), for 10 minutes. at RT or 37 degree C.
6. Wash slide with PBS 1X.
7. Follow instructions for IHC/ICC.The diluted serums are also used as blocking agents in all immunoassays.
Other Notes
Small volumes of Serum reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Serum purchase
MBS258540 is a reagent targeting Serum. Also known as Bovine Serum.
Additional Details
NCBI/Uniprot data below describe general gene information for Serum. It may not necessarily be applicable to this product.
Protein Family
Serum albumin
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.