AP22342b-ev | BRAF V600E Antibody(C-Term) Biovalley

All lanes : Anti-BRAF Antibody(C-Term) at 1:2000 dilution Lane 1: Braf-600V -wild type Lane 2: Braf-600E -mutation Lane 3: Raji whole cell lysate Lane 4: Hela whole cell lysate Lane 5: A549 whole cell lysate Lane 6: T47D whole cell lysate Lane 7: MDA-MB-453 whole cell lysate Lysates/proteins at 10 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
AP22342b staining BRAF in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	WB, IHC-P, E
Primary Accession	P15056
Other Accession	Q04982, P34908, P28028, P00531, A7E3S4, P05625, P04049, Q99N57, Q5R5M7, P11345, P00532, P27966, P10533
Reactivity	Human
Predicted	Mouse, Rat, Chicken, Bovine
Host	Rabbit
Clonality	polyclonal
Isotype	Rabbit IgG
Calculated MW	84437 Da

Additional Information
Gene ID	673
Other Names	Serine/threonine-protein kinase B-raf, 2.7.11.1, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF, BRAF1, RAFB1
Target/Specificity	This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 576-606 amino acids from human.
Dilution	WB~~1:2000 IHC-P~~1:25
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	BRAF V600E Antibody(C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	BRAF (HGNC:1097)
Synonyms	BRAF1, RAFB1
Function	Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). Phosphorylates PFKFB2 (PubMed:36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179).
Cellular Location	Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.
Tissue Location	Brain and testis.

BRAF V600E Antibody(C-Term)