BRD4 TR-FRET, BioAssay™ Kit (BD1, Bromodomain, HUNK1, MCAP)

Kit comes in a convenient format with purified BRD4 (BD1) protein, ligands, Tb donor, dye-labeled acceptor, assay buffer and microtiter plate to perform 384 reactions.

The BRD4 (BD1) TR-FRET BioAssay™ Kit is designed to measure the inhibition of BRD4 (BD1) binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BRD4, substrate, and an inhibitor is incubated for sixty minutes. Then, the fluorescence intensity is measured using a fluorescence reader.

Applications:
Suitable for use in screening small molecular inhibitors for drug discovery and HTS applications. Other applications not tested.

Kit Components:
White, nonbinding, low volume, microtiter plate, 1x plate
BRD4, BD1 (49-170), 1x5ug
BET Bromodomain Ligand, 1x50ul
Non-acetylated Ligand 1, 1x15ul
Tb donor, 2x10ul
Dye-labeled acceptor, 2x10ul
3x BRD TR-FRET Assay Buffer 1, 1x4ml

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.