C Reactive Protein (CRP) Recombinant, Human

Recombinant protein corresponding to Phe17-Pro224 with N-Terminal His Tag from human CRP, expressed in E. coli. Swiss/UniProt Accession: P02741||Amino Acid Sequence:|FGQT DMSRKAFVFP KESDTSYVSL KAPLTKPLKA FTVCLHFYTE LSSTRGYSIF SYATKRQDNE ILIFWSKDIG YSFTVGGSEI LFEVPEVTVA PVHICTSWES ASGIVEFWVD GKPRVRKSLK KGYTVGAEAS IILGQEQDSF GGNFEGSQSL VGDIGNVNMW DFVLSPDEIN TIYLGGPFSP NVLNWRALKY EVQGEVFTKP QLWP||Isoelectric Point:|6.3||Predicted Molecular Mass:|26.5kD||Accurate Molecular Mass:|27kD as determined by SDS-PAGE under reducing conditions||Subcellular Location:|Secreted||Applications:|Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.||Recommended Dilutions:|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. ||Note:|Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.