Cadherin, pan (APC)

Cadherins are a multifunctional family of Ca2+ -dependent, transmembrane glycoproteins which promote and maintain cell adhesion in virtually all multicellular organisms. The cadherin superfamily comprises over forty proteins which are, on average, 50-60% homologous (1). Cadherin expression is required for the assembly of cells into solid tissues and importantly, cadherins are expressed in a tissue specific fashion (2) . Homotypic cellular interactions are promoted by homophillic interactions between the extracellular regions of identical cadherin molecules on neighboring cells. Recent crystal structure analysis of an extracellular cadherin domain suggests that individual cadherin molecules cooperate to form a linear cell adhesion zipper (3) . In adherens junctions, cadherins are anchored to the actin cytoskeleton by interaction with the small cytoplasmic proteins b-catenin and g-catenin which both bind to the actin binding protein a-catenin (4,5) . The interaction of b-catenin with the cytoplasmic tail of cadherins and other cytoplasmic proteins, including Tcf-family transcription factors and the tumor suppressor protein APC, is thought to be mediated through a region of the b-catenin molecule containing multiple repeats of the 42 amino acid armadillo sequence motif (6). In addition to playing important roles in differentiation and tissue morphogenesis, cadherins also appear to play a significant role in modulating tumor invasion and metastasis (7). The expression of E-cadherin correlates inversely with the motile and invasive behavior of tumor cells. In addition, the tissue specificity of cadherin subtypes are becoming valuable markers for the identification and differential diagnosis of certain cancers (8,9).

Applications:
Suitable for use in FLISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1-10ug/ml. For Formalin fixed, paraffin embedded sections, Heat Induced Epitope Retrieval (HIER) step is required prior to staining.
Western Blot: 1ug/ml
FLISA: 0.1-1ug/ml

Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.