CAPN2 Antibody
Référence MBS9231851-0,1mL
Conditionnement : 0.1mL
Marque : MyBiosource
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
Western blot analysis of lysates from A549,SK-BR-3,mouse C2C12 cell line (from left to right), using CAPN2 Antibody (MBS9231851). It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
Flow cytometric analysis of U-87 MG cells using CAPN2 Antibody(green) (MBS9231851) compared to an isotype control of mouse IgG2b(blue). AP20600c was diluted at 1:100 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded H.stomach section using CAPN2 Antibody (MBS9231851) . It was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
CAPN2 Antibody – Mouse Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9231851Description: Purified Mouse Monoclonal Antibody (Mab)Background: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. More details
Product Overview
Online Datasheet in PDFProduct Name
CAPN2, Monoclonal Antibody
Full Product Name
CAPN2 Antibody
Product Gene Name
anti-CAPN2 antibody
Product Synonym Names
Calpain-2 catalytic subunit; Calcium-activated neutral proteinase 2; CANP 2; Calpain M-type; Calpain large polypeptide L2; Calpain-2 large subunit; Millimolar-calpain; M-calpain; CAPN2; CANPL2
Clonality
Monoclonal
Isotype
IgG2b
Host
Mouse
Species Reactivity
Human, Rat
Predicted: Mouse
Predicted: Mouse
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Specificity
This CAPN2 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from the human region of human CAPN2.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Molecular Weight
Calculated MW: H=80, 71; M=80, 71kDa
OMIM
114230
Cellular Location
Cytoplasm. Cell membrane.
Note=Translocates to the plasma membrane
upon Ca(2+) binding
Note=Translocates to the plasma membrane
upon Ca(2+) binding
Tissue Location
Ubiquitous.
Antigen Source
Human
Function
Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (PubMed:17650508). Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs (By similarity).
Applications Tested/Suitable for anti-CAPN2 antibody
Western Blot (WB), Immunohistochemistry Paraffin (IHC-P), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-CAPN2 antibody
WB ~~ 1:1000
FC/FACS ~~ 1:100
IHC-P ~~ 1:25
FC/FACS ~~ 1:100
IHC-P ~~ 1:25
Flow Cytometry (FC/FACS) of anti-CAPN2 antibody
Flow cytometric analysis of U-87 MG cells using CAPN2 Antibody(green) (MBS9231851) compared to an isotype control of mouse IgG2b(blue). AP20600c was diluted at 1:100 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Immunohistochemistry (IHC)-Paraffin of anti-CAPN2 antibody
Immunohistochemical analysis of paraffin-embedded H.stomach section using CAPN2 Antibody (MBS9231851) . It was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Product References and Citations for anti-CAPN2 antibody
Imajoh S.,et al.Biochemistry 27:8122-8128(1988). Ye Z.,et al.Biochem. Biophys. Res. Commun. 275:223-227(2000). Ota T.,et al.Nat. Genet. 36:40-45(2004). Gregory S.G.,et al.Nature 441:315-321(2006). Hata A.,et al.J. Biol. Chem. 264:6404-6411(1989).
Other Notes
Small volumes of anti-CAPN2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CAPN2 purchase
MBS9231851 is an antibody targeting CAPN2. Also known as CAPN2 Antibody. Reactive in Human, Rat
Predicted: Mouse. Host: Mouse.
Predicted: Mouse. Host: Mouse.
Additional Details
NCBI/Uniprot data below describe general gene information for CAPN2. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
calpain-2 catalytic subunit isoform 2
NCBI Official Synonym Full Names
calpain 2
NCBI Official Symbol
CAPN2
NCBI Official Synonym Symbols
CANP2; mCANP; CANPL2; CANPml
UniProt Protein Name
Calpain-2 catalytic subunit
UniProt Synonym Protein Names
Calcium-activated neutral proteinase 2; CANP 2; Calpain M-type; Calpain large polypeptide L2; Calpain-2 large subunit; Millimolar-calpain
Protein Family
Calpain
UniProt Gene Name
CAPN2
UniProt Synonym Gene Names
CANPL2; CANP 2; M-calpain
UniProt Entry Name
CAN2_HUMAN
NCBI Protein Information
calpain-2 catalytic subunit
NCBI Summary for CAPN2
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Show more UniProt Comments for CAPN2
CAPN2: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Forms a heterodimer with a small (regulatory) subunit (CAPNS1). Ubiquitous. Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin. Belongs to the peptidase C2 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Protease; Motility/polarity/chemotaxis; EC 3.4.22.53
Chromosomal Location of Human Ortholog: 1q41-q42
Cellular Component: Golgi apparatus; cortical actin cytoskeleton; focal adhesion; endoplasmic reticulum; dendrite; cytoplasm; plasma membrane; pseudopodium; cytosol; nucleus; chromatin; lipid raft
Molecular Function: protein binding; protein heterodimerization activity; cytoskeletal protein binding; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding; cysteine-type peptidase activity
Biological Process: protein autoprocessing; blastocyst development; proteolysis involved in cellular protein catabolic process; response to hypoxia; proteolysis; myoblast fusion; regulation of cytoskeleton organization and biogenesis
Show more Protein type: Protease; Motility/polarity/chemotaxis; EC 3.4.22.53
Chromosomal Location of Human Ortholog: 1q41-q42
Cellular Component: Golgi apparatus; cortical actin cytoskeleton; focal adhesion; endoplasmic reticulum; dendrite; cytoplasm; plasma membrane; pseudopodium; cytosol; nucleus; chromatin; lipid raft
Molecular Function: protein binding; protein heterodimerization activity; cytoskeletal protein binding; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding; cysteine-type peptidase activity
Biological Process: protein autoprocessing; blastocyst development; proteolysis involved in cellular protein catabolic process; response to hypoxia; proteolysis; myoblast fusion; regulation of cytoskeleton organization and biogenesis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with anti-CAPN2 antibody
| Reference Product | PubMed Publications |
|---|---|
| CAPNS1 antibody | >13 publications with CAPN2 and CAPNS1 |
| CAST antibody | >4 publications with CAPN2 and CAST |
| SRC antibody | >2 publications with CAPN2 and SRC |
| MAPK3 antibody | >1 publications with CAPN2 and MAPK3 |
Pathways associated with anti-CAPN2 antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| Alzheimer's Disease Pathway antibodies | Alzheimer's Disease Pathway Diagram |
| Alzheimer's Disease Pathway antibodies | Alzheimer's Disease Pathway Diagram |
| Alzheimers Disease Pathway antibodies | Alzheimers Disease Pathway Diagram |
| Apoptosis Pathway antibodies | Apoptosis Pathway Diagram |
| Apoptosis Pathway antibodies | Apoptosis Pathway Diagram |
| ErbB1 Downstream Signaling Pathway antibodies | ErbB1 Downstream Signaling Pathway Diagram |
| Focal Adhesion Pathway antibodies | Focal Adhesion Pathway Diagram |
| Focal Adhesion Pathway antibodies | Focal Adhesion Pathway Diagram |
| Integrin-mediated Cell Adhesion Pathway antibodies | Integrin-mediated Cell Adhesion Pathway Diagram |
| Protein Processing In Endoplasmic Reticulum Pathway antibodies | Protein Processing In Endoplasmic Reticulum Pathway Diagram |
Diseases associated with anti-CAPN2 antibody
| Disease Name | Pubmed Publications |
|---|---|
| Neoplasms Antibodies | >14 publications with CAPN2 and Neoplasms |
| Inflammation Antibodies | >6 publications with CAPN2 and Inflammation |
| Nervous System Diseases Antibodies | >5 publications with CAPN2 and Nervous System Diseases |
| Breast Neoplasms Antibodies | >4 publications with CAPN2 and Breast Neoplasms |
| Heart Diseases Antibodies | >3 publications with CAPN2 and Heart Diseases |
| Adenocarcinoma Antibodies | >3 publications with CAPN2 and Adenocarcinoma |
| Cardiovascular Diseases Antibodies | >3 publications with CAPN2 and Cardiovascular Diseases |
| Liver Neoplasms Antibodies | >2 publications with CAPN2 and Liver Neoplasms |
| Carcinoma, Hepatocellular Antibodies | >2 publications with CAPN2 and Carcinoma, Hepatocellular |
| Ovarian Neoplasms Antibodies | >2 publications with CAPN2 and Ovarian Neoplasms |
Organs/Tissues associated with anti-CAPN2 antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Muscle Antibodies | >15 publications with CAPN2 and Muscle |
| Embryonic Tissue Antibodies | >7 publications with CAPN2 and Embryonic Tissue |
| Brain Antibodies | >7 publications with CAPN2 and Brain |
| Nerve Antibodies | >5 publications with CAPN2 and Nerve |
| Lung Antibodies | >4 publications with CAPN2 and Lung |
| Liver Antibodies | >3 publications with CAPN2 and Liver |
| Bone Antibodies | >3 publications with CAPN2 and Bone |
| Heart Antibodies | >3 publications with CAPN2 and Heart |
| Skin Antibodies | >2 publications with CAPN2 and Skin |
| Testis Antibodies | >2 publications with CAPN2 and Testis |