Cas13a (LwC2c2), Active

Référence MBS516769-1mg

Conditionnement : 1mg

Marque : MyBiosource

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Cas13a (Lwc2c2) (CAS13A) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS516769

Recombinant full-length Leptotrichia wadei Cas13a protein with a His tag expressed in E. coli. More details

Product Overview

Online Datasheet in PDF
Product Name
Cas13a (LwC2c2), Recombinant Protein
Full Product Name
Cas13a (LwC2c2), Active
Product Gene Name
Cas13a recombinant protein
Product Synonym Names
LwCas13a; LwaCas13a; LwC2c2; EndoRNase; CRISPR-associated endoribonuclease Cas13a
Host
E Coli
Form/Format
Protein stored in 25mM Tris, 300mM NaCl, pH 7.5, 0.1mM TCEP, 50% glycerol
Purity/Purification
The purity was determined to be >90% by densitometry
Preparation and Storage
Store at -20°C. To avoid repeated handling and multiple freeze/thaw cycles aliquot diluted product into smaller quantities.
Molecular Weight
Calculated: 139.8 kDa
Observed: ~125 kDa
Source
Leptotrichia wadei
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for Cas13a recombinant protein

COVID-19 Proteins; Acute Respiratory Distress Syndrome; Cardiovascular Disease; Cellular Stress; COVID19; Gastrointestinal Diseases; Infectious Diseases; Inflammation; Lung Diseases; Metabolic Disorder; Neurobiology; severe acute respiratory syndrome coro

SDS-PAGE (SDS) of Cas13a recombinant protein

CAS13A Recombinant Protein – Cat# MBS516769

Activity of Cas13a recombinant protein

LwCas13a nuclease activity assayed using a fluorogenic substrate and 2nM of enzyme as per the Cas13 Activity Assay Protocol provided below.
Recommendation : Always include RNase inhibitor at recommended concentrations in Cas enzyme reactions
CAS13A Recombinant Protein, Activity – Cat# MBS516769

Product References and Citations for Cas13a recombinant protein

1. East-Seletsky A, et al: Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. Nature. 2016, 538:270-3.
2. Abudayyeh O, et al: C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Science. 2016, 353:5573.
3. Patchsung, M, et al: Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA. Nat Biomed Eng. 2020. https://doi.org/10.1038/s41551-020-00603-x.
4. Zhang J, You Y. CRISPR-Cas13a system: A novel approach to precision oncology. Cancer Biol Med. 2020, 17(1):6-8.

Other Notes

Small volumes of Cas13a recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Cas13a purchase

MBS516769 is a recombinant protein targeting Cas13a. Also known as Cas13a (LwC2c2), Active. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for Cas13a. It may not necessarily be applicable to this product.

NCBI Accession #

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

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