Catenin, beta, active (ABC) (APC)
Référence C2069-47-APC-100ul
Conditionnement : 100ul
Marque : US Biological
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C2069-47-APCCatenin, beta, active (ABC) (APC)
Specifications
Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin and beta-catenin. alpha-catenin can bind to beta-catenin and can also bind actin. beta-catenin binds the cytoplasmic domain of some cadherins. When beta-catenin was sequenced it was found to be a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain which is specialized for protein-protein binding. An increase in beta-catenin production has been noted in those people who have Basal Cell Carcinoma and leads to the increase in proliferation of related tumors. When beta-catenin is not associated with cadherins and alpha-catenin, it can interact with other proteins such as Catenin Beta Interacting Protein 1 (ICAT) and adenomatosis polyposis coli (APC). Recent evidence suggests that beta-catenin plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.
Applications:
Suitable for use in Immunohistochemistry, Immunocytochemistry, Flow Cytometry and Western Blot. Other applications not tested.
Recommended Dilutions:
Immunohistochemisty (Paraffin): Requires HIER using sodium citrate buffer pH6.0
Optimal dilutions to be determined by the researcher.
Positive Antigen Control Included:
A0001-50: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer.
Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.