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> Catenin, beta (HRP)

Catenin, beta (HRP)

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Référence C2069-50D-HRP-100ul

Conditionnement : 100ul

Marque : US Biological

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C2069-50D-HRPCatenin, beta (HRP)

Specifications
Clone TypePolyclonal
Hostrabbit
Sourcehuman
Swiss ProtP35222
IsotypeIgG
GradeAffinity Purified
ApplicationsE IP WB
CrossreactivityCh Hu Mo Rt Xe
Shipping TempBlue Ice
Storage Temp-20°C

Applications:
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
ELISA: 0.1-1.0ug/ml
Western Blot after IP: 1-2ug/ml. Crossreactivity with a ~100kD protein of unknown identity is sometimes observed.
Immunoprecipitation: 5-10ug. Specifically immunoprecipitate the ~ 92kD b-Catenin protein from native cell lysates.
Optimal dilutions to be determined by the researcher.

Positive Controls:
HeLa and A431 lysates.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: human|Concentration: As reported|Form: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with horseradish peroxidase (HRP).|Purity: Purified by immunoaffinity chromatography.|Immunogen: A 15 amino acid synthetic peptide derived from the C-terminus of the human and mouse b-catenin protein. |Specificity: Recognizes human b-catenin. Species Crossreactivity: chicken, mouse, rat and Xenopus..||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
A 15 amino acid synthetic peptide derived from the C-terminus of the human and mouse b-catenin protein.
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with horseradish peroxidase (HRP).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human b-catenin. Species Crossreactivity: chicken, mouse, rat and Xenopus..
References
1. Johnson, K.R., et al; Exp. Cell Res. 207:252–260 (1993). 2. Geiger, B., et al; Curr. Opin. Cell Biol. 1:103–109 (1989). 3. Peifer, M., et al; Science 262:1667–1668 (1993). 4. Takeichi, M., et al; Science 51:1451–1455 (1991). 5. Daniel, J.M., et al; Mol. Cell. Biol. 270:4819–4824 (1995). 6. Hinck, L, et al; J. Cell. Biol. 125:1327–1340 (1994). 7. Knudsen, K.A., et al; J. Cell. Biol. 18:671–679 (1992). 8. McCrea, P.D., et al; J. Biol. Chem. 266:4514–4520 (1991). 9. Ozawa, M., et al; Proc. Natl Acad. Sci. USA. 87:4246–4250 (1990). 10. Knudsen, K.A., et al; J. Biol. Chem. 130:67–77 (1995). 11. Ozawa, M., et al; J. Cell. Biol. 116:989–996 (1992). 12. McCrea, P.D., et al; Science 25:1359–1361 (1991). 13. Peifer, M., et al; Cell 63:1167–1178 (1990). 14. Peifer, M., et al; Cell 76:789–791 (1994). 15. Rubinfeld, B., et al; Science 262:1731–1734 (1993). 16. Su, L.-K., et al; Science 262:1734–1737 (1993). 17. Kowalczk, A.P., et al; J. Biol. Chem. 269:31214–31223 (1994)