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Product Description
CD1 Mouse Bone Marrow Dendritic Cells from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory CD1 mice. Cells at passage 0 are cryo-preserved and each vial contains 2x106 cells per ml. CD1 Mouse Bone Marrow Dendritic Cells are characterized by immunofluorescence staining with antibody of CD11c. These cells will not proliferate in culture and cannot be passaged.
Storage
Cryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.
Authorized Uses of Cell Biologics’ Products
CD1 Mouse Bone Marrow Dendritic Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.
Disclaimer
Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Cell Biologics’
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Primary Cell Culture Protocol
All cell culture procedures must be conducted in a biosafety cabinet.
Any and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.
Use aseptic technique to prevent microbial contamination.
Cryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.
Medium: Use pre-warmed (37°C) cell culture media of Catalog No. M7711 (30-50 ML) to recover cryopreserved cells and when changing media or splitting cells.
Cell recovery from cryovial:
- Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for <1 min until there is just a small bit of ice left in the vial.
- Promptly remove the vial and wipe it down with 70% ethanol.
- Transfer cells from the vial to a sterile centrifuge tube. Add 8-10 ml of pre-warmed Cell Biologics Cell Culture Medium.
- Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.
- Centrifuge cells at 200 x g for 5 minutes.
- Aspirate the supernatant and resuspend the cell pellet in Cell Biologics’ Cell Culture Growth medium.
- Proceed with designed experiments.