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> CD11c (Monocyte, Macrophage, Integrin alphaX, ITGAX, SLEB6) (FITC)

CD11c (Monocyte, Macrophage, Integrin alphaX, ITGAX, SLEB6) (FITC)

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Référence C2262-57L-100T

Conditionnement : 100Tests

Marque : US Biological

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C2262-57LCD11c (Monocyte, Macrophage, Integrin alphaX, ITGAX, SLEB6) (FITC)

Specifications
Clone TypeMonoclonal
Hostmouse
Sourcehuman
Swiss ProtP20702
IsotypeIgG1
GradePurified
ApplicationsFC
CrossreactivityHu Mk
Shipping TempBlue Ice
Storage Temp-20°C

CD11c (p150, alphaX integrin subunit) forms complex with CD18 (beta2 integrin subunit) and is expressed mainly on tissue macrophages and dendritic cells. CD11c binds to complement fragment iC3b, fibrinogen, VCAM-1 and ICAM-2 or e.g. CD90. Like other beta2 integrins, CD11c/CD18 plays roles in cell migration and phagocytosis. Moreover, interaction of CD11c/CD18 with plasminogen regulates plasmin activities, and interaction with heparin counteracts binding of iC3b.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 20ul labels 100ul of whole blood or 10e6 cells in a suspension
Optimal working dilutions to be determined by researcher.

Excitation:
Blue (488nm)

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: BU15|Host: mouse|Source: human|Concentration: Not Determined |Form: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with Fluorescein isothiocyanate (FITC). |Purity: Purified|Immunogen: Dendritic cells of synovial fluid|Specificity: Recognizes human CD11c. Species Crossreactivity: monkey||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Dendritic cells of synovial fluid
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified
Specificity
Recognizes human CD11c. Species Crossreactivity: monkey
References
1. Hogg, N., Takacs, L., Palmer, D.G., Selvendran, Y. and Allen, C. (1986). The p150, 95 molecule is a marker of human mononuclear phagocytes: comparison with expression of class II molecules. Eur. J. Immunol. 16: 240.|2. Van der Vieren M, Le Trong H, Wood CL, Moore PF, St John T, Staunton DE, Gallatin WM: A novel leukointegrin, alpha d beta 2, binds preferentially to ICAM-3. Immunity. 1995 Dec;3(6):683-90.|3. Sadhu C, Hendrickson L, Dick KO, Potter TG, Staunton DE: Novel Tools for Functional Analysis of CD11c: Activation-Specific, Activation-Independent, and Activating Antibodies. J Immunoassay Immunochem. 2008;29(1):42-57.|4. Rezzonico R, Imbert V, Chicheportiche R, Dayer JM: Ligation of CD11b and CD11c beta(2) integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1alpha (MIP-1alpha) and MIP-1beta production in primary human monocytes through a pathway dependent on nuclear factor-kappaB. Blood. 2001 May 15;97(10):2932-40.|5. Myou S, Zhu X, Boetticher E, Qin Y, Myo S, Meliton A, Lambertino A, Munoz NM, Hamann KJ, Leff AR: Regulation of adhesion of AML14.3D10 cells by surface clustering of beta2-integrin caused by ERK-independent activation of cPLA2. Immunology. 2002 Sep;107(1):77-85.