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> CD11R1 (HRP)

CD11R1 (HRP)

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Référence C2262-90-HRP-100ul

Conditionnement : 100ul

Marque : US Biological

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C2262-90-HRPCD11R1 (HRP)

Specifications
Clone TypeMonoclonal
Hostmouse
Sourceporcine
IsotypeIgG1
GradeAffinity Purified
ApplicationsIHC IP
CrossreactivityGp Hu Po
Shipping TempBlue Ice
Storage Temp-20°C

recognizes the porcine cell surface antigen classified as CD11R1 at the Third International Workshop on Swine Leukocyte Differentiation Antigens (Haverson et al. 2001). Clone MIL4 stains porcine eosinophils, a subset of neutrophils and NK cells, it does not stain monocytes or macrophages (Haverson et al. 1994).

Applications:
Suitable for use in Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Hybridoma:
P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 10B652 (MIL4)|Host: mouse|Source: porcine|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).|Purity: Purified by Protein A affinity chromatography from tissue culture supernatant.|Immunogen: Porcine Lamina Propria Leukocytes.|Specificity: Recognizes porcine CD11R1. Species Crossreactivity: human and guinea pig||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Porcine Lamina Propria Leukocytes.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes porcine CD11R1. Species Crossreactivity: human and guinea pig
References
1. Haverson, K. et al. (1994) Characterisation of monoclonal antibodies specific for monocytes, macrophages and granulocytes from porcine peripheral blood and mucosal tissue. J. Immunol. Methods 170: 233-245, 2. Dominguez, J. et al. (2001) Workshop studies on monoclonal antibodies in the myeloid panel with CD11 specificity. Vet. Immunol. Immunopathol. 80: 111-119. 3. Inman, C.F. et al. (2010) Dendritic cells interact with CD4 T cells in intestinal mucosa. J Leukoc Biol. 88: 571-8. 4. Cheng, Q. et al. (2010) Administered CpG oligodeoxynucleotide induces mRNA expression of CXC and CC chemokines at the intestinal mucosa and PBMCs in piglets. Int Immunopharmacol. 10: 611-8. 5. Ordway, D.et al. (2007) The cellular immune response to Mycobacterium tuberculosis infection in the guinea pig. J Immunol. 179: 2532-41. 6. Shang, S. et al. (2010) The activity of TMC 207, rifampin, and pyrazinamide against Mycobacterium tuberculosis infection in guinea pigs. Antimicrob Agents Chemother. 55: 124-31. 8. Rank, R.G. et al. (2012) Effect of Inflammatory Response on In Vivo Competition between Two Chlamydial Variants in the Guinea Pig Model of Inclusion Conjunctivitis. Infect Immun. 80: 612-9. 9. Suda, Y. et al. (2014) Immunobiotic Lactobacillus jensenii as immune-health promoting factor to improve growth performance and productivity in post-weaning pigs. BMC Immunol. 15: 24.